Enhancement of androgen transcriptional activation assay based on genome edited glucocorticoid knock out human prostate cancer cell line

Hee Seok Lee; Seok Hee Lee; Yooheon Park

doi:10.1016/j.envres.2019.01.027

Enhancement of androgen transcriptional activation assay based on genome edited glucocorticoid knock out human prostate cancer cell line

Hee Seok Lee
, Seok Hee Lee
, Yooheon Park

Department of Food Science & Biotechnology

National Institute of Food and Drug Safety Evaluation

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Endocrine-disrupting chemicals (EDCs) interfere with the biological activity of hormones. Among EDC's, (anti-)androgenic compounds potentially cause several androgen-related diseases. To improve the accuracy of an in vitro transactivation assay (TA) for detection of (anti-)androgenic compounds, We established the glucocorticoid receptor (GR) knockout 22Rv1/MMTV cell line by using an RNA-guided engineered nuclease (RGEN)-derived CRISPR/Cas system. The 22Rv1/MMTV GRKO cell line was characterized and validated by androgen receptor (AR)-mediated TA assay compared with the AR-TA assay using 22Rv1/MMTV. In conclusion, the AR-TA assay with the 22Rv1/MMTV GRKO cell line was more accurate, excluding the misleading signals derived from glucocorticoids or equivalent chemicals, and might be an effective method for screening potential (anti-)androgenic compounds.

Original language	English
Pages (from-to)	437-443
Number of pages	7
Journal	Environmental Research
Volume	171
DOIs	https://doi.org/10.1016/j.envres.2019.01.027
State	Published - Apr 2019

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

22Rv1 cell lines
Androgen transactivation assay
Glucocorticoid receptor
RNA-guided engineered nuclease

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10.1016/j.envres.2019.01.027

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title = "Enhancement of androgen transcriptional activation assay based on genome edited glucocorticoid knock out human prostate cancer cell line",

abstract = "Endocrine-disrupting chemicals (EDCs) interfere with the biological activity of hormones. Among EDC's, (anti-)androgenic compounds potentially cause several androgen-related diseases. To improve the accuracy of an in vitro transactivation assay (TA) for detection of (anti-)androgenic compounds, We established the glucocorticoid receptor (GR) knockout 22Rv1/MMTV cell line by using an RNA-guided engineered nuclease (RGEN)-derived CRISPR/Cas system. The 22Rv1/MMTV GRKO cell line was characterized and validated by androgen receptor (AR)-mediated TA assay compared with the AR-TA assay using 22Rv1/MMTV. In conclusion, the AR-TA assay with the 22Rv1/MMTV GRKO cell line was more accurate, excluding the misleading signals derived from glucocorticoids or equivalent chemicals, and might be an effective method for screening potential (anti-)androgenic compounds.",

keywords = "22Rv1 cell lines, Androgen transactivation assay, Glucocorticoid receptor, RNA-guided engineered nuclease",

author = "Lee, \{Hee Seok\} and Lee, \{Seok Hee\} and Yooheon Park",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",

year = "2019",

month = apr,

doi = "10.1016/j.envres.2019.01.027",

language = "English",

volume = "171",

pages = "437--443",

journal = "Environmental Research",

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}

TY - JOUR

T1 - Enhancement of androgen transcriptional activation assay based on genome edited glucocorticoid knock out human prostate cancer cell line

AU - Lee, Hee Seok

AU - Lee, Seok Hee

AU - Park, Yooheon

PY - 2019/4

Y1 - 2019/4

N2 - Endocrine-disrupting chemicals (EDCs) interfere with the biological activity of hormones. Among EDC's, (anti-)androgenic compounds potentially cause several androgen-related diseases. To improve the accuracy of an in vitro transactivation assay (TA) for detection of (anti-)androgenic compounds, We established the glucocorticoid receptor (GR) knockout 22Rv1/MMTV cell line by using an RNA-guided engineered nuclease (RGEN)-derived CRISPR/Cas system. The 22Rv1/MMTV GRKO cell line was characterized and validated by androgen receptor (AR)-mediated TA assay compared with the AR-TA assay using 22Rv1/MMTV. In conclusion, the AR-TA assay with the 22Rv1/MMTV GRKO cell line was more accurate, excluding the misleading signals derived from glucocorticoids or equivalent chemicals, and might be an effective method for screening potential (anti-)androgenic compounds.

AB - Endocrine-disrupting chemicals (EDCs) interfere with the biological activity of hormones. Among EDC's, (anti-)androgenic compounds potentially cause several androgen-related diseases. To improve the accuracy of an in vitro transactivation assay (TA) for detection of (anti-)androgenic compounds, We established the glucocorticoid receptor (GR) knockout 22Rv1/MMTV cell line by using an RNA-guided engineered nuclease (RGEN)-derived CRISPR/Cas system. The 22Rv1/MMTV GRKO cell line was characterized and validated by androgen receptor (AR)-mediated TA assay compared with the AR-TA assay using 22Rv1/MMTV. In conclusion, the AR-TA assay with the 22Rv1/MMTV GRKO cell line was more accurate, excluding the misleading signals derived from glucocorticoids or equivalent chemicals, and might be an effective method for screening potential (anti-)androgenic compounds.

KW - 22Rv1 cell lines

KW - Androgen transactivation assay

KW - Glucocorticoid receptor

KW - RNA-guided engineered nuclease

UR - https://www.scopus.com/pages/publications/85060988982

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DO - 10.1016/j.envres.2019.01.027

M3 - Article

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AN - SCOPUS:85060988982

SN - 0013-9351

VL - 171

SP - 437

EP - 443

JO - Environmental Research

JF - Environmental Research

ER -

Enhancement of androgen transcriptional activation assay based on genome edited glucocorticoid knock out human prostate cancer cell line

Abstract

UN SDGs

Keywords

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