Enhancement of matrix metalloproteinase-2 (MMP-2) as a potential chondrogenic marker during chondrogenic differentiation of human adipose-derived stem cells

Yoshie Arai, Sunghyun Park, Bogyu Choi, Kyoung Won Ko, Won Chul Choi, Joong Myung Lee, Dong Wook Han, Hun Kuk Park, Inbo Han, Jong Hun Lee, Soo Hong Lee

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Human adipose-derived stem cells (hASCs) have a capacity to undergo adipogenic, chondrogenic, and osteogenic differentiation. Recently, hASCs were applied to various fields including cell therapy for tissue regeneration. However, it is hard to predict the direction of differentiation of hASCs in real-time. Matrix metalloproteinases (MMPs) are one family of proteolytic enzymes that plays a pivotal role in regulating the biology of stem cells. MMPs secreted by hASCs are expected to show different expression patterns depending on the differentiation state of hASCs because biological functions exhibit different patterns during the differentiation of stem cells. Here, we investigated proteolytic enzyme activity, especially MMP-2 activity, in hASCs during their differentiation. The activities of proteolytic enzymes and MMP-2 were higher during chondrogenic differentiation than during adipogenic and osteogenic differentiation. During chondrogenic differentiation, mRNA expression of MMP-2 and the level of the active form of MMP-2 were increased, which also correlated with Col II. It is concluded that proteolytic enzyme activity and the level of the active form of MMP-2 were increased during chondrogenic differentiation, which was accelerated in the presence of Col II protein. According to our findings, MMP-2 could be a candidate maker for real-time detection of chondrogenic differentiation of hASCs.

Original languageEnglish
Article number963
JournalInternational Journal of Molecular Sciences
Volume17
Issue number6
DOIs
StatePublished - Jun 2016

Keywords

  • Chondrogenic differentiation
  • Extracellular matrix
  • Human adipose-derived stem cells
  • Matrix metalloproteinase-2

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