TY - JOUR
T1 - Enhancing Proliferation and ECM Expression of Human ACL Fibroblasts by Sonic Vibration
AU - Jiang, Yuan Yuan
AU - Park, Jung Keug
AU - Yoon, Hee Hoon
AU - Choi, Hynjin
AU - Kim, Chan Wha
AU - Seo, Young Kwon
N1 - Publisher Copyright:
© 2015 Taylor and Francis Group, LLC.
PY - 2015/7/4
Y1 - 2015/7/4
N2 - Effects of mechanical vibration on cell activity and behavior remain controversial: There has been evidence on both positive and negative effects. Furthermore, research on the anterior cruciate ligament (ACL) has as yet been limited and the frequency-related effects remain unknown, even though ACL injury is common and an injuredACL hardly spontaneously recovers. The object of this work was to address the influence of mechanical vibration on ACL fibroblasts, to determine the effects of frequencies, and to further study this effect at the cellular level. We found that sonic vibration affectedACL fibroblasts' proliferation and metabolism in a frequency-dependent manner, and 20 Hz gave rise to the most ACL cell activity and comprehensively increased extracellular matrix (ECM) contents, including collagen type I, collagen type III, fibronectin, elastin, tenascin, glycosaminoglycan (GAG), and the cytoskeleton protein vimentin. Thus, our results indicate that sonic vibration possesses frequency-dependent effects on proliferation and productivity of ACL fibroblast with an optimal frequency of 20 Hz under the present stimulation conditions, providing further information for future research in how vibrational stimulation manipulates ACL cellular behavior.
AB - Effects of mechanical vibration on cell activity and behavior remain controversial: There has been evidence on both positive and negative effects. Furthermore, research on the anterior cruciate ligament (ACL) has as yet been limited and the frequency-related effects remain unknown, even though ACL injury is common and an injuredACL hardly spontaneously recovers. The object of this work was to address the influence of mechanical vibration on ACL fibroblasts, to determine the effects of frequencies, and to further study this effect at the cellular level. We found that sonic vibration affectedACL fibroblasts' proliferation and metabolism in a frequency-dependent manner, and 20 Hz gave rise to the most ACL cell activity and comprehensively increased extracellular matrix (ECM) contents, including collagen type I, collagen type III, fibronectin, elastin, tenascin, glycosaminoglycan (GAG), and the cytoskeleton protein vimentin. Thus, our results indicate that sonic vibration possesses frequency-dependent effects on proliferation and productivity of ACL fibroblast with an optimal frequency of 20 Hz under the present stimulation conditions, providing further information for future research in how vibrational stimulation manipulates ACL cellular behavior.
KW - Extracellular matrix
KW - human anterior cruciate ligament fibroblast
KW - proliferation
KW - sonic vibration
UR - http://www.scopus.com/inward/record.url?scp=84908375426&partnerID=8YFLogxK
U2 - 10.1080/10826068.2014.923444
DO - 10.1080/10826068.2014.923444
M3 - Article
C2 - 24842289
AN - SCOPUS:84908375426
SN - 1082-6068
VL - 45
SP - 476
EP - 490
JO - Preparative Biochemistry and Biotechnology
JF - Preparative Biochemistry and Biotechnology
IS - 5
ER -