TY - JOUR
T1 - Ensifer collicola sp. nov., a bacterium isolated from soil in South Korea
AU - Jang, Jun Hyeong
AU - Lee, Dongwook
AU - Cha, Seho
AU - Seo, Taegun
N1 - Publisher Copyright:
© 2017, The Microbiological Society of Korea and Springer-Verlag GmbH Germany.
PY - 2017/7/1
Y1 - 2017/7/1
N2 - Strain Mol12T, which presented in the form of Gram-negative, motile, non-spore forming rod-shaped, was isolated from soil in South Korea and characterized to determine its taxonomic position. The strain grew at 20–30°C (optimum 30°C) and pH 7.0–10.0 (optimum pH 8.0) with 1% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain Mol12T was most closely related to Ensifer terangae LMG 7834T (96.78%), Rhizobium daejeonense KCTC 12121T (96.43%), Ensifer adhaerens Casida AT (96.28%). Chemotaxonomic data showed that the predominant fatty acids were Summed Feature 8 (C18:1ω7c and/or C18:1ω6c; 53.02%) and C18:1ω7c 11-methyl (24.01%). Its complex polar lipid contained major amounts of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) and Q-10 as the predominant ubiquinone. The DNA G+C content of strain Mol12T was determined to be 60.9 mol%. Based on the phylogenetic, chemotaxonomic, and phenotypic data, strain Mol12T (=KCTC 42816T =JCM 31049T) ought to be classified as a type strain of a novel species, for which the name Ensifer collicola sp. nov. is proposed.
AB - Strain Mol12T, which presented in the form of Gram-negative, motile, non-spore forming rod-shaped, was isolated from soil in South Korea and characterized to determine its taxonomic position. The strain grew at 20–30°C (optimum 30°C) and pH 7.0–10.0 (optimum pH 8.0) with 1% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain Mol12T was most closely related to Ensifer terangae LMG 7834T (96.78%), Rhizobium daejeonense KCTC 12121T (96.43%), Ensifer adhaerens Casida AT (96.28%). Chemotaxonomic data showed that the predominant fatty acids were Summed Feature 8 (C18:1ω7c and/or C18:1ω6c; 53.02%) and C18:1ω7c 11-methyl (24.01%). Its complex polar lipid contained major amounts of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) and Q-10 as the predominant ubiquinone. The DNA G+C content of strain Mol12T was determined to be 60.9 mol%. Based on the phylogenetic, chemotaxonomic, and phenotypic data, strain Mol12T (=KCTC 42816T =JCM 31049T) ought to be classified as a type strain of a novel species, for which the name Ensifer collicola sp. nov. is proposed.
KW - Ensifer
KW - Ensifer collicola sp. nov
KW - Rhizobiaceae
KW - taxonomy
UR - http://www.scopus.com/inward/record.url?scp=85021677201&partnerID=8YFLogxK
U2 - 10.1007/s12275-017-6608-z
DO - 10.1007/s12275-017-6608-z
M3 - Article
C2 - 28664521
AN - SCOPUS:85021677201
SN - 1225-8873
VL - 55
SP - 520
EP - 524
JO - Journal of Microbiology
JF - Journal of Microbiology
IS - 7
ER -