Epstein–Barr Virus Promotes Gastric Cancer Progression by Modulating m6A-Dependent YTHDF1–TSC22D1 Axis

Yea Rim An; Jaehun Jung; Kyeong Min Kwon; Jun Yeob Kim; Min Hyeok Lee; Ju Yeon Lee; Minho Lee; Suk Kyeong Lee

doi:10.3390/microorganisms13122820

Epstein–Barr Virus Promotes Gastric Cancer Progression by Modulating m6A-Dependent YTHDF1–TSC22D1 Axis

Yea Rim An
, Jaehun Jung
, Kyeong Min Kwon
, Jun Yeob Kim
, Min Hyeok Lee
, Ju Yeon Lee
, Minho Lee
, Suk Kyeong Lee

Department of Life Science

Research output: Contribution to journal › Article › peer-review

Abstract

Epstein–Barr virus (EBV) infection is closely associated with gastric cancer, yet its role in m6A-dependent gene regulation remains poorly understood. In this study, we investigated how EBV infection alters the m6A methylation pattern in gastric cancer cells and examined its impact on TSC22D1 mRNA stability through interaction with the m6A reader protein YTHDF1. m6A RNA immunoprecipitation sequencing (MeRIP-seq) revealed a significant reduction in m6A methylation of TSC22D1 in EBV-infected gastric cancer cells (AGS-EBV) compared with EBV-negative cells (AGS). Moreover, YTHDF1 knockdown increased both the stability and expression of TSC22D1. These findings demonstrate that YTHDF1 binds to TSC22D1 mRNA and promotes its m6A-dependent degradation. Collectively, our results suggest that EBV infection modulates m6A modification to regulate gene stability and identify the YTHDF1–TSC22D1 axis as a potential therapeutic target in EBV-associated gastric cancer.

Original language	English
Article number	2820
Journal	Microorganisms
Volume	13
Issue number	12
DOIs	https://doi.org/10.3390/microorganisms13122820
State	Published - Dec 2025

Keywords

Epstein–Barr virus
TSC22D1
YTHDF1
epitranscriptomics
gastric cancer
m6A modification

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/microorganisms13122820

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@article{b014be2083234f929ca18abd093d39e1,

title = "Epstein–Barr Virus Promotes Gastric Cancer Progression by Modulating m6A-Dependent YTHDF1–TSC22D1 Axis",

abstract = "Epstein–Barr virus (EBV) infection is closely associated with gastric cancer, yet its role in m6A-dependent gene regulation remains poorly understood. In this study, we investigated how EBV infection alters the m6A methylation pattern in gastric cancer cells and examined its impact on TSC22D1 mRNA stability through interaction with the m6A reader protein YTHDF1. m6A RNA immunoprecipitation sequencing (MeRIP-seq) revealed a significant reduction in m6A methylation of TSC22D1 in EBV-infected gastric cancer cells (AGS-EBV) compared with EBV-negative cells (AGS). Moreover, YTHDF1 knockdown increased both the stability and expression of TSC22D1. These findings demonstrate that YTHDF1 binds to TSC22D1 mRNA and promotes its m6A-dependent degradation. Collectively, our results suggest that EBV infection modulates m6A modification to regulate gene stability and identify the YTHDF1–TSC22D1 axis as a potential therapeutic target in EBV-associated gastric cancer.",

keywords = "Epstein–Barr virus, TSC22D1, YTHDF1, epitranscriptomics, gastric cancer, m6A modification",

author = "An, \{Yea Rim\} and Jaehun Jung and Kwon, \{Kyeong Min\} and Kim, \{Jun Yeob\} and Lee, \{Min Hyeok\} and Lee, \{Ju Yeon\} and Minho Lee and Lee, \{Suk Kyeong\}",

note = "Publisher Copyright: {\textcopyright} 2025 by the authors.",

year = "2025",

month = dec,

doi = "10.3390/microorganisms13122820",

language = "English",

volume = "13",

journal = "Microorganisms",

issn = "2076-2607",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

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T1 - Epstein–Barr Virus Promotes Gastric Cancer Progression by Modulating m6A-Dependent YTHDF1–TSC22D1 Axis

AU - An, Yea Rim

AU - Jung, Jaehun

AU - Kwon, Kyeong Min

AU - Kim, Jun Yeob

AU - Lee, Min Hyeok

AU - Lee, Ju Yeon

AU - Lee, Minho

AU - Lee, Suk Kyeong

PY - 2025/12

Y1 - 2025/12

N2 - Epstein–Barr virus (EBV) infection is closely associated with gastric cancer, yet its role in m6A-dependent gene regulation remains poorly understood. In this study, we investigated how EBV infection alters the m6A methylation pattern in gastric cancer cells and examined its impact on TSC22D1 mRNA stability through interaction with the m6A reader protein YTHDF1. m6A RNA immunoprecipitation sequencing (MeRIP-seq) revealed a significant reduction in m6A methylation of TSC22D1 in EBV-infected gastric cancer cells (AGS-EBV) compared with EBV-negative cells (AGS). Moreover, YTHDF1 knockdown increased both the stability and expression of TSC22D1. These findings demonstrate that YTHDF1 binds to TSC22D1 mRNA and promotes its m6A-dependent degradation. Collectively, our results suggest that EBV infection modulates m6A modification to regulate gene stability and identify the YTHDF1–TSC22D1 axis as a potential therapeutic target in EBV-associated gastric cancer.

AB - Epstein–Barr virus (EBV) infection is closely associated with gastric cancer, yet its role in m6A-dependent gene regulation remains poorly understood. In this study, we investigated how EBV infection alters the m6A methylation pattern in gastric cancer cells and examined its impact on TSC22D1 mRNA stability through interaction with the m6A reader protein YTHDF1. m6A RNA immunoprecipitation sequencing (MeRIP-seq) revealed a significant reduction in m6A methylation of TSC22D1 in EBV-infected gastric cancer cells (AGS-EBV) compared with EBV-negative cells (AGS). Moreover, YTHDF1 knockdown increased both the stability and expression of TSC22D1. These findings demonstrate that YTHDF1 binds to TSC22D1 mRNA and promotes its m6A-dependent degradation. Collectively, our results suggest that EBV infection modulates m6A modification to regulate gene stability and identify the YTHDF1–TSC22D1 axis as a potential therapeutic target in EBV-associated gastric cancer.

KW - Epstein–Barr virus

KW - TSC22D1

KW - YTHDF1

KW - epitranscriptomics

KW - gastric cancer

KW - m6A modification

UR - https://www.scopus.com/pages/publications/105025973811

U2 - 10.3390/microorganisms13122820

DO - 10.3390/microorganisms13122820

M3 - Article

AN - SCOPUS:105025973811

SN - 2076-2607

VL - 13

JO - Microorganisms

JF - Microorganisms

IS - 12

M1 - 2820

ER -

Epstein–Barr Virus Promotes Gastric Cancer Progression by Modulating m6A-Dependent YTHDF1–TSC22D1 Axis

Abstract

Keywords

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