TY - JOUR
T1 - Establishment of a rapid micropropagation system for kaempferia parviflora wall. Ex baker
T2 - Phytochemical analysis of leaf extracts and evaluation of biological activities
AU - Park, Han Yong
AU - Kim, Kyung Su
AU - Ak, Gunes
AU - Zengin, Gokhan
AU - Cziáky, Zoltán
AU - Jekő, József
AU - Adaikalam, Kathalingam
AU - Song, Kihwan
AU - Kim, Doo Hwan
AU - Sivanesan, Iyyakkannu
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/4
Y1 - 2021/4
N2 - This study aimed to establish a rapid in vitro plant regeneration method from rhizome buds of Kaempferia parviflora to obtain the valuable secondary metabolites with antioxidant and enzyme inhibition properties. The disinfection effect of silver oxide nanoparticles (AgO NPs) on rhizome and effects of plant growth regulators on shoot multiplication and subsequent rooting were investigated. Surface sterilization of rhizome buds with sodium hypochlorite was insufficient to control contamination. However, immersing rhizome buds in 100 mg L−1 AgO NPs for 60 min elim-inated contamination without affecting the survival of explants. The number of shoots (12.2) pro-duced per rhizome bud was higher in Murashige and Skoog (MS) medium containing 8 μM of 6‐ Benzyladenine (6‐BA) and 0.5 μM of Thidiazuron (TDZ) than other treatments. The highest number of roots (24), with a mean root length of 7.8 cm and the maximum shoot length (9.8 cm), were obtained on medium MS with 2 μM of Indole‐3‐butyric acid (IBA). A survival rate of 98% was attained when plantlets of K. parviflora were acclimatized in a growth room. Liquid chromatography with tandem mass spectrometry (LC‐MS/MS) was used to determine the chemical profile of K. parviflora leaf extracts. Results showed that several biologically active flavonoids reported in rhizomes were also present in leaf tissues of both in vitro cultured and ex vitro (greenhouse‐grown) plantlets of K. parviflora. We found 40 and 36 compounds in in vitro cultured and ex vitro grown leaf samples, respectively. Greenhouse leaves exhibited more potent antioxidant activities than leaves from in vitro cultures. A higher acetylcholinesterase inhibitory ability was obtained for greenhouse leaves (1.07 mg/mL). However, leaves from in vitro cultures exhibited stronger butyrylcholinesterase in-hibitory abilities. These results suggest that leaves of K. parviflora, as major byproducts of black ginger cultivation, could be used as valuable alternative sources for extracting bioactive compounds.
AB - This study aimed to establish a rapid in vitro plant regeneration method from rhizome buds of Kaempferia parviflora to obtain the valuable secondary metabolites with antioxidant and enzyme inhibition properties. The disinfection effect of silver oxide nanoparticles (AgO NPs) on rhizome and effects of plant growth regulators on shoot multiplication and subsequent rooting were investigated. Surface sterilization of rhizome buds with sodium hypochlorite was insufficient to control contamination. However, immersing rhizome buds in 100 mg L−1 AgO NPs for 60 min elim-inated contamination without affecting the survival of explants. The number of shoots (12.2) pro-duced per rhizome bud was higher in Murashige and Skoog (MS) medium containing 8 μM of 6‐ Benzyladenine (6‐BA) and 0.5 μM of Thidiazuron (TDZ) than other treatments. The highest number of roots (24), with a mean root length of 7.8 cm and the maximum shoot length (9.8 cm), were obtained on medium MS with 2 μM of Indole‐3‐butyric acid (IBA). A survival rate of 98% was attained when plantlets of K. parviflora were acclimatized in a growth room. Liquid chromatography with tandem mass spectrometry (LC‐MS/MS) was used to determine the chemical profile of K. parviflora leaf extracts. Results showed that several biologically active flavonoids reported in rhizomes were also present in leaf tissues of both in vitro cultured and ex vitro (greenhouse‐grown) plantlets of K. parviflora. We found 40 and 36 compounds in in vitro cultured and ex vitro grown leaf samples, respectively. Greenhouse leaves exhibited more potent antioxidant activities than leaves from in vitro cultures. A higher acetylcholinesterase inhibitory ability was obtained for greenhouse leaves (1.07 mg/mL). However, leaves from in vitro cultures exhibited stronger butyrylcholinesterase in-hibitory abilities. These results suggest that leaves of K. parviflora, as major byproducts of black ginger cultivation, could be used as valuable alternative sources for extracting bioactive compounds.
KW - Antioxidant activity
KW - Enzyme inhibition
KW - Flavonoids
KW - Kaempferia parviflora
KW - Micropropagation
KW - Silver oxide nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85103516134&partnerID=8YFLogxK
U2 - 10.3390/plants10040698
DO - 10.3390/plants10040698
M3 - Article
AN - SCOPUS:85103516134
SN - 2223-7747
VL - 10
JO - Plants
JF - Plants
IS - 4
M1 - 698
ER -