Ethanol extract of Aster glehni exhibits anti-inflammatory and anti-oxidant effects in RAW 264.7 cells and Caenorhabditis elegans

Mi Kyung Seo, Han Na Chu, Da Bin Lee, Haeng Ran Kim, In Seon Hwang, Yong Jin Jeong, Sung Ran Yoon, Seok Seong Kang, Kyeong A. Jang, Min Sook Kang

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

This study investigated the anti-oxidative and anti-inflammatory effects of Aster glehni (AG) extract in RAW 264.7 cells and Caenorhabditis elegans. The total polyphenol and flavonoid contents were higher in the ethanol extracts than in the hot water extracts. As a result of measuring the moisture contents (%) and extraction yields (%) of AG and drying A. glehni for processing (DAG), 70% ethanol, which has the highest percentage of extraction yield, was selected as the final solvent. DPPH radical scavenging activity showed higher antioxidant activity of ethanol extracts of DAG than AG. The cytotoxicity assay of the AG or DAG ethanol extracts was treated at different concentrations (25, 50, and 100 μg/mL), and cell viability rates were higher than 80% at all concentrations. The LPS-stimulated nitric oxide (NO) production in RAW 264.7 was significantly reduced at all concentrations of AG and DAG groups. As a result of measuring the gene expression of iNOS, which induces NO production, the AG or DAG group decreased by 33% and 32%, compared with the phosphate buffer saline (PBS) group. Under inflammatory stress conditions, the survival rate of C. elegans treated with AG or DAG ethanol extract with LPS showed concentration-dependent improvement in survival rate compared with the PBS group. Considering these results, AG could potentially be developed as an antioxidant and anti-inflammatory functional food material.

Original languageEnglish
Pages (from-to)1095-1106
Number of pages12
JournalKorean Journal of Food Preservation
Volume30
Issue number6
DOIs
StatePublished - 2023

Keywords

  • agrofood processing
  • anti-inflammatory
  • Aster glehni
  • Caenorhabditis elegans
  • food-tech

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