Exposure to salinity and light spectra regulates glucosinolates, phenolics, and antioxidant capacity of brassica carinata l. Microgreens

Sylvia Maina, Da Hye Ryu, Jwa Yeong Cho, Da Seul Jung, Jai Eok Park, Chu Won Nho, Gaymary Bakari, Gerald Misinzo, Je Hyeong Jung, Seung Hoon Yang, Ho Youn Kim

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The effect of salt treatment on Brassica carinata (BC) microgreens grown under different light wavelengths on glucosinolates (GLs) and phenolic compounds were evaluated. Quantifiable GLs were identified using ultra-high performance-quadrupole time of flight mass spectrometry. Extracts’ ability to activate antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)) was evaluated on human colorectal carcinoma cells (HCT116). Furthermore, BC compounds’ ability to activate expression of nuclear transcription factor-erythroid 2 related factor (Nrf2) and heme-oxygenase-1 (HO-1) proteins was examined using specific antibodies on HCT116 cells. Sinigrin (SIN) was the abundant GLs of the six compounds identified and its content together with total aliphatic GLs increased in saline conditions. Fluorescent (FL) and blue plus red (B1R1) lights were identified as stable cultivation conditions for microgreens, promoting biomass and glucobrassicin contents, whereas other identified individual and total indole GLs behaved differently in saline and non-saline environments. Blue light-emitting diodes and FL light in saline treatments mostly enhanced SIN, phenolics and antioxidant activities. The increased SOD and CAT activities render the BC microgreens suitable for lowering oxidative stress. Additionally, activation of Nrf2, and HO-1 protein expression by the GLs rich extracts, demonstrate their potential to treat and prevent oxidative stress and inflammatory disorders. Therefore, effective salt treatments and light exposure to BC microgreens present an opportunity for targeted regulation of growth and accumulation of bioactive metabolites.

Original languageEnglish
Article number1183
JournalAntioxidants
Volume10
Issue number8
DOIs
StatePublished - Aug 2021

Keywords

  • Antioxidant enzymes
  • Antioxidant proteins
  • Bioactive compounds
  • Brassicaceae
  • Light wavelength
  • Oxidative stress
  • Reactive oxygen species (ROS)

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