TY - JOUR
T1 - Fabrication of chitosan/fibrin-armored multifunctional silver nanocomposites to improve antibacterial and wound healing activities
AU - Sanmugam, Anandhavelu
AU - Shanthi, D.
AU - Sairam, Ananda Babu
AU - Kumar, Raju Suresh
AU - Almansour, Abdulrahman I.
AU - Arumugam, Natrajan
AU - Kavitha, A.
AU - Kim, Hyun Seok
AU - Vikraman, Dhanasekaran
N1 - Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2024/2
Y1 - 2024/2
N2 - A wound healing substitute promotes rapid tissue regeneration and protects wound sites from microbial contamination. The silver-based antiseptic frequently moist skin stains, burns and irritation, penetrates deep wounds and protects against pathogenic infections. Thus, we formulated a novel fibrin/chitosan encapsulated silver nanoparticle (CH:F:SPG-CH:SNP) composites bandage accelerating the polymicrobial wound healing. Electrospinning method was employed to form the nano-porous, inexpensive, and biocompatible smart bandages. The structural, functional, and mechanical properties were analyzed for the prepared composites. The biological capacity of prepared CH:F:SPG-CH:SNP bandage was assessed against NIH-3 T3 fibroblast and HaCaT cell lines. In vitro hemolytic assays using red blood cells were extensively studied and explored the low hemolytic effect (4.5 %). In addition, the improved drug delivery nature captured for the CH:F:SPG-CH:SNP composite bandage. Antibacterial experiments were achieved against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Lactobacillus bulgaricus using zone inhibition method. Moreover, in-vivo wound healing efficacy of fabricated smart bandage was evaluated on the albino Wistar rats which revealed the significant improvement on the postoperative abdomen wounds.
AB - A wound healing substitute promotes rapid tissue regeneration and protects wound sites from microbial contamination. The silver-based antiseptic frequently moist skin stains, burns and irritation, penetrates deep wounds and protects against pathogenic infections. Thus, we formulated a novel fibrin/chitosan encapsulated silver nanoparticle (CH:F:SPG-CH:SNP) composites bandage accelerating the polymicrobial wound healing. Electrospinning method was employed to form the nano-porous, inexpensive, and biocompatible smart bandages. The structural, functional, and mechanical properties were analyzed for the prepared composites. The biological capacity of prepared CH:F:SPG-CH:SNP bandage was assessed against NIH-3 T3 fibroblast and HaCaT cell lines. In vitro hemolytic assays using red blood cells were extensively studied and explored the low hemolytic effect (4.5 %). In addition, the improved drug delivery nature captured for the CH:F:SPG-CH:SNP composite bandage. Antibacterial experiments were achieved against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Lactobacillus bulgaricus using zone inhibition method. Moreover, in-vivo wound healing efficacy of fabricated smart bandage was evaluated on the albino Wistar rats which revealed the significant improvement on the postoperative abdomen wounds.
KW - Antibacterial
KW - Chitosan
KW - Wound healing
UR - http://www.scopus.com/inward/record.url?scp=85178999899&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2023.128598
DO - 10.1016/j.ijbiomac.2023.128598
M3 - Article
C2 - 38056742
AN - SCOPUS:85178999899
SN - 0141-8130
VL - 257
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
M1 - 128598
ER -