TY - JOUR
T1 - Fluorescein derived Schiff base as fluorimetric zinc (II) sensor via ‘turn on’ response and its application in live cell imaging
AU - Das, Bhriguram
AU - Jana, Atanu
AU - Mahapatra, Ananya Das
AU - Chattopadhyay, Debprasad
AU - Dhara, Anamika
AU - Mabhai, Subhabrata
AU - Dey, Satyajit
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/4/5
Y1 - 2019/4/5
N2 - A novel Schiff base L composed of fluorescein hydrazine and a phenol functionalized moiety has been designed and prepared via cost-effective condensation reaction. The L is utilized for selective sensing of Zn 2+ over other environmental and biological relevant metal ions in aqueous alcoholic solution under physiological pH range. The binding of Zn 2+ to the receptor L is found to causes ~23 fold fluorescence enhancement of L. The 1:1 binding mode of the metal complex is established by combined UV–Vis, fluorescence, and HRMS (high-resolution mass spectroscopy) spectroscopic methods. The binding constant (K a ) for complexation and the limit of detection (LOD) of Zn 2+ is calculated to be 2.86 × 10 4 M −1 and 1.59 μM, respectively. Further photophysical investigations including steady-state, time-resolved fluorescence analysis and spectral investigations including NMR (nuclear magnetic resonance), IR (infrared spectroscopy) suggest introduction of CHEF (chelation enhance fluorescence) with the suppression of C[dbnd]N isomerization and PET (photo-induced electron transfer) mechanism for the strong fluorescent response towards Zn 2+ . Finally, the sensor L is successfully employed to monitor a real-time detection of Zn 2+ by means of TLC (thin layer chromatography) based paper strip. The L is used in the cell imaging study using African green monkey kidney cells (Vero cells) for the determination of exogenous Zn 2+ by Immunofluorescence Assay (IFA) process.
AB - A novel Schiff base L composed of fluorescein hydrazine and a phenol functionalized moiety has been designed and prepared via cost-effective condensation reaction. The L is utilized for selective sensing of Zn 2+ over other environmental and biological relevant metal ions in aqueous alcoholic solution under physiological pH range. The binding of Zn 2+ to the receptor L is found to causes ~23 fold fluorescence enhancement of L. The 1:1 binding mode of the metal complex is established by combined UV–Vis, fluorescence, and HRMS (high-resolution mass spectroscopy) spectroscopic methods. The binding constant (K a ) for complexation and the limit of detection (LOD) of Zn 2+ is calculated to be 2.86 × 10 4 M −1 and 1.59 μM, respectively. Further photophysical investigations including steady-state, time-resolved fluorescence analysis and spectral investigations including NMR (nuclear magnetic resonance), IR (infrared spectroscopy) suggest introduction of CHEF (chelation enhance fluorescence) with the suppression of C[dbnd]N isomerization and PET (photo-induced electron transfer) mechanism for the strong fluorescent response towards Zn 2+ . Finally, the sensor L is successfully employed to monitor a real-time detection of Zn 2+ by means of TLC (thin layer chromatography) based paper strip. The L is used in the cell imaging study using African green monkey kidney cells (Vero cells) for the determination of exogenous Zn 2+ by Immunofluorescence Assay (IFA) process.
KW - Cell imaging
KW - Molecular logic gate
KW - PL and TCSPC
KW - Schiff base
KW - UV–Vis
KW - Zn sensor
UR - http://www.scopus.com/inward/record.url?scp=85059750615&partnerID=8YFLogxK
U2 - 10.1016/j.saa.2018.12.053
DO - 10.1016/j.saa.2018.12.053
M3 - Article
C2 - 30641362
AN - SCOPUS:85059750615
SN - 1386-1425
VL - 212
SP - 222
EP - 231
JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
ER -