Fluorescein derived Schiff base as fluorimetric zinc (II) sensor via ‘turn on’ response and its application in live cell imaging

Bhriguram Das, Atanu Jana, Ananya Das Mahapatra, Debprasad Chattopadhyay, Anamika Dhara, Subhabrata Mabhai, Satyajit Dey

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

A novel Schiff base L composed of fluorescein hydrazine and a phenol functionalized moiety has been designed and prepared via cost-effective condensation reaction. The L is utilized for selective sensing of Zn 2+ over other environmental and biological relevant metal ions in aqueous alcoholic solution under physiological pH range. The binding of Zn 2+ to the receptor L is found to causes ~23 fold fluorescence enhancement of L. The 1:1 binding mode of the metal complex is established by combined UV–Vis, fluorescence, and HRMS (high-resolution mass spectroscopy) spectroscopic methods. The binding constant (K a ) for complexation and the limit of detection (LOD) of Zn 2+ is calculated to be 2.86 × 10 4 M −1 and 1.59 μM, respectively. Further photophysical investigations including steady-state, time-resolved fluorescence analysis and spectral investigations including NMR (nuclear magnetic resonance), IR (infrared spectroscopy) suggest introduction of CHEF (chelation enhance fluorescence) with the suppression of C[dbnd]N isomerization and PET (photo-induced electron transfer) mechanism for the strong fluorescent response towards Zn 2+ . Finally, the sensor L is successfully employed to monitor a real-time detection of Zn 2+ by means of TLC (thin layer chromatography) based paper strip. The L is used in the cell imaging study using African green monkey kidney cells (Vero cells) for the determination of exogenous Zn 2+ by Immunofluorescence Assay (IFA) process.

Original languageEnglish
Pages (from-to)222-231
Number of pages10
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume212
DOIs
StatePublished - 5 Apr 2019

Keywords

  • Cell imaging
  • Molecular logic gate
  • PL and TCSPC
  • Schiff base
  • UV–Vis
  • Zn sensor

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