Free radical initiated peptide sequencing using MALDI-TOF/TOF mass spectrometry

Insu Song; Jae Ung Lee; Jaehyeon Baek; Sangwon Cha; Sang Yun Han; Han Bin Oh

doi:10.5478/MSL.2018.9.2.56

Free radical initiated peptide sequencing using MALDI-TOF/TOF mass spectrometry

Insu Song, Jae Ung Lee, Jaehyeon Baek, Sangwon Cha, Sang Yun Han, Han Bin Oh

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

In this study, matrix-assisted laser desorption/ionization (MALDI) was applied to the TEMPO-assisted FRIPS for the first time. We found that 3-HPA is the optimal matrix for the analysis of p-TEMPO-Bz-Sc-peptides, which gives minimal precursor fragmentations. MALDI-TOF/TOF experiments on p-TEMPO-Bz-Sc-peptides yielded mainly [a_n+H]⁺, [z_n+H]⁺, and [y_n]⁺-type products, indicating that radical-driven peptide fragmentation occurs in MALDI-TOF/TOF-MS.

Original language	English
Pages (from-to)	56-60
Number of pages	5
Journal	Mass Spectrometry Letters
Volume	9
Issue number	2
DOIs	https://doi.org/10.5478/MSL.2018.9.2.56
State	Published - Jun 2018

Keywords

FRIPS
MALDI-TOF/TOF
Mass spectrometry
Peptides
Radical ion
Radical-driven fragmentation

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10.5478/MSL.2018.9.2.56

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title = "Free radical initiated peptide sequencing using MALDI-TOF/TOF mass spectrometry",

abstract = "In this study, matrix-assisted laser desorption/ionization (MALDI) was applied to the TEMPO-assisted FRIPS for the first time. We found that 3-HPA is the optimal matrix for the analysis of p-TEMPO-Bz-Sc-peptides, which gives minimal precursor fragmentations. MALDI-TOF/TOF experiments on p-TEMPO-Bz-Sc-peptides yielded mainly [an+H]+, [zn+H]+, and [yn]+-type products, indicating that radical-driven peptide fragmentation occurs in MALDI-TOF/TOF-MS.",

keywords = "FRIPS, MALDI-TOF/TOF, Mass spectrometry, Peptides, Radical ion, Radical-driven fragmentation",

author = "Insu Song and Lee, {Jae Ung} and Jaehyeon Baek and Sangwon Cha and Han, {Sang Yun} and Oh, {Han Bin}",

note = "Publisher Copyright: {\textcopyright} Korean Society for Mass Spectrometry.",

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doi = "10.5478/MSL.2018.9.2.56",

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AU - Song, Insu

AU - Lee, Jae Ung

AU - Baek, Jaehyeon

AU - Cha, Sangwon

AU - Han, Sang Yun

AU - Oh, Han Bin

N1 - Publisher Copyright: © Korean Society for Mass Spectrometry.

PY - 2018/6

Y1 - 2018/6

N2 - In this study, matrix-assisted laser desorption/ionization (MALDI) was applied to the TEMPO-assisted FRIPS for the first time. We found that 3-HPA is the optimal matrix for the analysis of p-TEMPO-Bz-Sc-peptides, which gives minimal precursor fragmentations. MALDI-TOF/TOF experiments on p-TEMPO-Bz-Sc-peptides yielded mainly [an+H]+, [zn+H]+, and [yn]+-type products, indicating that radical-driven peptide fragmentation occurs in MALDI-TOF/TOF-MS.

AB - In this study, matrix-assisted laser desorption/ionization (MALDI) was applied to the TEMPO-assisted FRIPS for the first time. We found that 3-HPA is the optimal matrix for the analysis of p-TEMPO-Bz-Sc-peptides, which gives minimal precursor fragmentations. MALDI-TOF/TOF experiments on p-TEMPO-Bz-Sc-peptides yielded mainly [an+H]+, [zn+H]+, and [yn]+-type products, indicating that radical-driven peptide fragmentation occurs in MALDI-TOF/TOF-MS.

KW - FRIPS

KW - MALDI-TOF/TOF

KW - Mass spectrometry

KW - Peptides

KW - Radical ion

KW - Radical-driven fragmentation

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M3 - Article

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SN - 2233-4203

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SP - 56

EP - 60

JO - Mass Spectrometry Letters

JF - Mass Spectrometry Letters

IS - 2

ER -

Free radical initiated peptide sequencing using MALDI-TOF/TOF mass spectrometry

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