Free radical initiated peptide sequencing using MALDI-TOF/TOF mass spectrometry

Insu Song; Jae Ung Lee; Jaehyeon Baek; Sangwon Cha; Sang Yun Han; Han Bin Oh

doi:10.5478/MSL.2018.9.2.56

Free radical initiated peptide sequencing using MALDI-TOF/TOF mass spectrometry

Insu Song
, Jae Ung Lee
, Jaehyeon Baek
, Sangwon Cha
, Sang Yun Han
, Han Bin Oh

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

In this study, matrix-assisted laser desorption/ionization (MALDI) was applied to the TEMPO-assisted FRIPS for the first time. We found that 3-HPA is the optimal matrix for the analysis of p-TEMPO-Bz-Sc-peptides, which gives minimal precursor fragmentations. MALDI-TOF/TOF experiments on p-TEMPO-Bz-Sc-peptides yielded mainly [a_n+H]⁺, [z_n+H]⁺, and [y_n]⁺-type products, indicating that radical-driven peptide fragmentation occurs in MALDI-TOF/TOF-MS.

Original language	English
Pages (from-to)	56-60
Number of pages	5
Journal	Mass Spectrometry Letters
Volume	9
Issue number	2
DOIs	https://doi.org/10.5478/MSL.2018.9.2.56
State	Published - Jun 2018

Keywords

FRIPS
MALDI-TOF/TOF
Mass spectrometry
Peptides
Radical ion
Radical-driven fragmentation

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10.5478/MSL.2018.9.2.56

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title = "Free radical initiated peptide sequencing using MALDI-TOF/TOF mass spectrometry",

abstract = "In this study, matrix-assisted laser desorption/ionization (MALDI) was applied to the TEMPO-assisted FRIPS for the first time. We found that 3-HPA is the optimal matrix for the analysis of p-TEMPO-Bz-Sc-peptides, which gives minimal precursor fragmentations. MALDI-TOF/TOF experiments on p-TEMPO-Bz-Sc-peptides yielded mainly [an+H]+, [zn+H]+, and [yn]+-type products, indicating that radical-driven peptide fragmentation occurs in MALDI-TOF/TOF-MS.",

keywords = "FRIPS, MALDI-TOF/TOF, Mass spectrometry, Peptides, Radical ion, Radical-driven fragmentation",

author = "Insu Song and Lee, \{Jae Ung\} and Jaehyeon Baek and Sangwon Cha and Han, \{Sang Yun\} and Oh, \{Han Bin\}",

note = "Publisher Copyright: {\textcopyright} Korean Society for Mass Spectrometry.",

year = "2018",

month = jun,

doi = "10.5478/MSL.2018.9.2.56",

language = "English",

volume = "9",

pages = "56--60",

journal = "Mass Spectrometry Letters",

issn = "2233-4203",

publisher = "Korean Society Mass Spectrometry",

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TY - JOUR

T1 - Free radical initiated peptide sequencing using MALDI-TOF/TOF mass spectrometry

AU - Song, Insu

AU - Lee, Jae Ung

AU - Baek, Jaehyeon

AU - Cha, Sangwon

AU - Han, Sang Yun

AU - Oh, Han Bin

N1 - Publisher Copyright: © Korean Society for Mass Spectrometry.

PY - 2018/6

Y1 - 2018/6

N2 - In this study, matrix-assisted laser desorption/ionization (MALDI) was applied to the TEMPO-assisted FRIPS for the first time. We found that 3-HPA is the optimal matrix for the analysis of p-TEMPO-Bz-Sc-peptides, which gives minimal precursor fragmentations. MALDI-TOF/TOF experiments on p-TEMPO-Bz-Sc-peptides yielded mainly [an+H]+, [zn+H]+, and [yn]+-type products, indicating that radical-driven peptide fragmentation occurs in MALDI-TOF/TOF-MS.

AB - In this study, matrix-assisted laser desorption/ionization (MALDI) was applied to the TEMPO-assisted FRIPS for the first time. We found that 3-HPA is the optimal matrix for the analysis of p-TEMPO-Bz-Sc-peptides, which gives minimal precursor fragmentations. MALDI-TOF/TOF experiments on p-TEMPO-Bz-Sc-peptides yielded mainly [an+H]+, [zn+H]+, and [yn]+-type products, indicating that radical-driven peptide fragmentation occurs in MALDI-TOF/TOF-MS.

KW - FRIPS

KW - MALDI-TOF/TOF

KW - Mass spectrometry

KW - Peptides

KW - Radical ion

KW - Radical-driven fragmentation

UR - https://www.scopus.com/pages/publications/85050026569

U2 - 10.5478/MSL.2018.9.2.56

DO - 10.5478/MSL.2018.9.2.56

M3 - Article

AN - SCOPUS:85050026569

SN - 2233-4203

VL - 9

SP - 56

EP - 60

JO - Mass Spectrometry Letters

JF - Mass Spectrometry Letters

IS - 2

ER -

Free radical initiated peptide sequencing using MALDI-TOF/TOF mass spectrometry

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