Abstract
Vibrio cholerae O1 employs the ATP-binding cassette (ABC) transporter-dependent pathway for O antigen biosynthesis. Different from highly studied Klebsiella pneumoniae and Escherichia coli, it was reported that initial reaction of O antigen biosynthesis in V. cholerae O1 may be involved in WbeW protein, which is predicted to be a galactosyltransferase. In this work, we report expression and characterization of WbeW enzyme. WbeW was expressed as membrane-associated form in E. coli and it was obtained with high purity. The enzyme had a function of transferring Gal-1-P from UDP-Gal to Und-P, implying that initial glycan of O antigen in V. cholerae O1 can be composed of a Gal residue.
Original language | English |
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Pages (from-to) | 980-987 |
Number of pages | 8 |
Journal | Biotechnology and Bioprocess Engineering |
Volume | 20 |
Issue number | 6 |
DOIs | |
State | Published - 1 Nov 2015 |
Keywords
- membrane protein
- undecaprenyl phosphate galactose-1-phosphate transferase
- Vibrio cholerae O1
- WbeW enzyme