Functional characterization of Vibrio cholerae O1 WbeW enzyme responsible for initial reaction in O antigen biosynthesis

Chang Sup Kim, Jeong Hyun Seo, Hyung Joon Cha

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Vibrio cholerae O1 employs the ATP-binding cassette (ABC) transporter-dependent pathway for O antigen biosynthesis. Different from highly studied Klebsiella pneumoniae and Escherichia coli, it was reported that initial reaction of O antigen biosynthesis in V. cholerae O1 may be involved in WbeW protein, which is predicted to be a galactosyltransferase. In this work, we report expression and characterization of WbeW enzyme. WbeW was expressed as membrane-associated form in E. coli and it was obtained with high purity. The enzyme had a function of transferring Gal-1-P from UDP-Gal to Und-P, implying that initial glycan of O antigen in V. cholerae O1 can be composed of a Gal residue.

Original languageEnglish
Pages (from-to)980-987
Number of pages8
JournalBiotechnology and Bioprocess Engineering
Volume20
Issue number6
DOIs
StatePublished - 1 Nov 2015

Keywords

  • membrane protein
  • undecaprenyl phosphate galactose-1-phosphate transferase
  • Vibrio cholerae O1
  • WbeW enzyme

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