Gene expression profile of human peripheral blood mononuclear cells induced by Staphylococcus aureus lipoteichoic acid

Seok Seong Kang, Hye Jin Kim, Mi Seon Jang, Sunjin Moon, Sang In Lee, Jun Ho Jeon, Jung Eun Baik, Ok Jin Park, Young Min Son, Gi Rak Kim, Donghyun Joo, Heebal Kim, Jae Yong Han, Cheol Heui Yun, Seung Hyun Han

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Lipoteichoic acid (LTA) is a major virulence factor of Gram-positive bacteria including Staphylococcus aureus. Despite its pivotal role in causing sepsis, the systemic immune responses to LTA in human cells are poorly understood. Here, we produced highly-pure and structurally-intact LTA from S. aureus and examined the gene expression profile of LTA-stimulated human peripheral blood mononuclear cells (PBMCs). The LTA preparation did not contain any detectable biologically-active impurities and stimulated Toll-like receptor 2. Protein expression profiling using a cytokine array kit and ELISA revealed expression of MCP-1/CCL2, IL-6, and IL-1β. We performed transcriptional profiling of PBMCs in response to S. aureus LTA using an Affymetrix genechip microarray. A total of 208 genes were significantly (fold change > 1.5 and P < 0.05) altered, with 157 up-regulated and 51 down-regulated genes in response to S. aureus LTA treatment. The up-regulated genes were involved in recognition (30 genes), cellular adhesion (6 genes), signal transduction (42 genes), co-stimulation (4 genes), chemokines, cytokines and their receptors (51 genes), apoptosis (9 genes), and negative regulation (15 genes). The down-regulated genes were involved in recognition (12 genes), antigen processing and presentation (9 genes), signal transduction (27 genes), and chemotaxis (3 genes). The microarray results were validated using real-time RT-PCR with 21 up-regulated genes and 9 down-regulated genes. Our results provide a more comprehensive overview of the transcriptional changes in PBMCs in response to S. aureus LTA, and contribute to the understanding of the pathophysiological role of S. aureus LTA during the systemic inflammatory response.

Original languageEnglish
Pages (from-to)454-460
Number of pages7
JournalInternational Immunopharmacology
Volume13
Issue number4
DOIs
StatePublished - Aug 2012

Keywords

  • Human peripheral blood mononuclear cells
  • Lipoteichoic acid
  • Microarray
  • Staphylococcus aureus
  • Systemic inflammatory response

Fingerprint

Dive into the research topics of 'Gene expression profile of human peripheral blood mononuclear cells induced by Staphylococcus aureus lipoteichoic acid'. Together they form a unique fingerprint.

Cite this