TY - JOUR
T1 - Glu-tRNA(Gln) amidotransferase
T2 - A novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation
AU - Curnow, A. W.
AU - Hong, K. W.
AU - Yuan, R.
AU - Kim, S. I.
AU - Martins, O.
AU - Winkler, W.
AU - Henkin, T. M.
AU - Soll, D.
PY - 1997
Y1 - 1997
N2 - The three genes, gatC, gatA, and gatB, which constitute the transcriptional unit of the Bacillus subtilis glutamyl-tRNA(Gln) amidotransferase have been cloned. Expression of this transcriptional unit results in the production of a heterotrimeric protein that has been purified to homogeneity. The enzyme furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln), functionally replacing the lack of glutaminyl-tRNA synthetase activity in Gram-positive eubacteria, cyanobacteria, Archaea, and organelles. Disruption of this operon is lethal. This demonstrates that transamidation is the only pathway to Gln-tRNA(Gln) in B. subtilis and that glutamyl-tRNA(Gln) amidotransferase is a novel and essential component of the translational apparatus.
AB - The three genes, gatC, gatA, and gatB, which constitute the transcriptional unit of the Bacillus subtilis glutamyl-tRNA(Gln) amidotransferase have been cloned. Expression of this transcriptional unit results in the production of a heterotrimeric protein that has been purified to homogeneity. The enzyme furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln), functionally replacing the lack of glutaminyl-tRNA synthetase activity in Gram-positive eubacteria, cyanobacteria, Archaea, and organelles. Disruption of this operon is lethal. This demonstrates that transamidation is the only pathway to Gln-tRNA(Gln) in B. subtilis and that glutamyl-tRNA(Gln) amidotransferase is a novel and essential component of the translational apparatus.
UR - http://www.scopus.com/inward/record.url?scp=0030613553&partnerID=8YFLogxK
U2 - 10.1073/pnas.94.22.11819
DO - 10.1073/pnas.94.22.11819
M3 - Article
C2 - 9342321
AN - SCOPUS:0030613553
SN - 0027-8424
VL - 94
SP - 11819
EP - 11826
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 22
ER -