Green Synthesis and Characterization of NiO Nanoparticles using Catharanthus Roseus Leaf Extract

Nanomaterials are primarily synthesized using chemical methods, which have inadvertent consequences to environmental damage, high energy utilization, and promising medical issues. Due to low cost, produces less pollution, improves environmental and human health safety, green synthesis is preferable traditionally. In the present work, Nickel Oxide Nanoparticles (NiO NPs) were reduced phytochemically, Eco-friendly, Cost effective using leaf of Catharanthus roseus. X-ray diffraction pattern, Fourier-transform infrared spectroscopy, ultra-violet visible spectroscopy, Dynamic Light Scattering scanning electron microscopy and energy-dispersive X-ray spectroscopy were utilized to analyze the prepared sample. The synthesized NPs revealed cubic structured pattern by XRD and strong absorption observed at 365 nm using UV–vis spectrophotometer. The formation of spherical shape NPs (50-60 nm) confirmed from SEM analysis. By increasing concentrations of the sample, phytochemically reduced NPs showed increased bactericidal action against Gram Positive Staphylococcus aureus and Gram negative Escherichia coli, resulting in an environmentally benign catalytic agent that could be used on an industrial scale. The dose dependent antioxidant activity was analyzed using DPPH assay.