Abstract
This study was conducted to test the hypothesis that growth factors can reduce the suppressive effect of titanium particles on MSCs. Cultured human MSCs at passage 3 were challenged with prepared cpTi particles at a concentration of 500 particles/cell along with one of the following growth factors: TGF-β1 (10 ng/mL), FGF-2 (10 ng/mL), IGF-I (100 ng/mL), and BMP-6 (50 ng/mL). After various periods of time, the treatment effects on cellular proliferation, viability, and osteogenic differentiation were measured. All the four growth factors positively promoted cell proliferation and viability to a varying extent. FGF-2 most effectively enhanced cell proliferation, whereas IGF-I was the most effective growth factor for enhancing cell viability. FGF-2, IGF-I, and BMP-6 reversed the titanium-mediated suppression of osteogenic differentiation, BMP-6 being the most effective one. Various growth factors can mitigate the suppressive effects of titanium particles on MSCs and enhance cell proliferation, viability, and osteogenic differentiation.
Original language | English |
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Pages (from-to) | 1137-1144 |
Number of pages | 8 |
Journal | Journal of Biomedical Materials Research - Part A |
Volume | 86 |
Issue number | 4 |
DOIs | |
State | Published - 15 Sep 2008 |
Keywords
- Arthroplasty
- Growth factor
- Mesenchymal stem cells
- Periprosthetic osteolysis
- Titanium