Abstract
Purpose: To report the expression of macrophage migration inhibitory factor (MIF) in murine lacrimal glands. Methods: Six lacrimal glands from 3 female 8-week-old CBA/J mice were analyzed using a gene microarray method (Oligo GEArray mouse inflammatory cytokines and receptors, Catalog No. OMM-011, SuperArray Bioscience) and immunofluorescent staining for MIF. Nine lacrimal glands from 8-week-old CBA/J mice, 9 lacrimal glands from 8-week-old C57BL/6 mice, and 7 lacrimal glands from 8-week-old BALB/c mice were analyzed using real-time reverse transcription-polymerase chain reaction. Five spleens, 5 livers, and 5 serum samples from 5 female 8-week-old CBA/J mice were analyzed using enzyme-linked immunosorbent assay. Results: Microarray analysis revealed that MIF is highly expressed in the murine lacrimal gland. Lacrimal acinar cells stain strongly with anti-MIF antibodies. Real-time reverse transcription- polymerase chain reaction revealed that the MIF mRNA expression level is lower in lacrimal glands of CBA/J mice compared with C57BL/6 and BALB/c mice when normalized against the expression of β-actin mRNA. Enzyme-linked immunosorbent assay revealed that MIF level was higher in lacrimal glands and spleens compared with livers and sera. Conclusions: The murine lacrimal gland expresses high levels of macrophage MIF without any evidence of lacrimal gland inflammation.
| Original language | English |
|---|---|
| Pages (from-to) | 187-191 |
| Number of pages | 5 |
| Journal | Cornea |
| Volume | 29 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2010 |
Keywords
- Dry eye
- Dysfunctional tear
- Inflammation
- Inhibition
- Lacrimal gland
- Macrophage
- MIF
- Mouse
- Ocular surface