TY - JOUR
T1 - Histidine functionalized gold nanoparticles for screening aminoglycosides and nanomolar level detection of streptomycin in water, milk, and whey
AU - Shinde, Surendra Krushna
AU - Kim, Dae Young
AU - Saratale, Rijuta Ganesh
AU - Kadam, Avinash Ashok
AU - Saratale, Ganesh Dattatraya
AU - Syed, Asad
AU - Bahkali, Ali H.
AU - Ghodake, Gajanan Sampatrao
N1 - Publisher Copyright:
© 2021, MDPI. All rights reserved.
PY - 2021/12
Y1 - 2021/12
N2 - Aminoglycoside (AMG) antibiotics are being applied to treat infections caused by Gram-negative bacteria, mainly in livestock, and are prescribed only in severe cases because of their adverse impacts on human health and the environment. Monitoring antibiotic residues in dairy products relies on the accessibility of portable and efficient analytical techniques. Presently, high-throughput screening techniques have been proposed to detect several antimicrobial drugs having identical structural and functional features. The L-histidine functionalized gold nanoparticles (His@AuNPs) do not form a complex with other tested antibiotic classes but show high selectivity for AMG antibiotics. We used ligand-induced aggregation of His@AuNPs as a rapid and sensitive localized surface plasmon resonance (LSPR) assay for AMG antibiotics, producing longitudinal extinction shifts at 660 nm. Herein, we explore the practical application of His@AuNPs to detect streptomycin spiked in water, milk, and whey fraction of milk with nanomolar level sensitivity. The ability of the analytical method to recognize target analytes sensitively and rapidly is of great significance to perform monitoring, thus would certainly reassure widespread use of AMG antibiotics. The biosynthesis of hybrid organic–inorganic metal nanoparticles like His@AuNPs with desired size distribution, stability, and specific host–guest recognition proficiency, would further facilitate applications in various other fields.
AB - Aminoglycoside (AMG) antibiotics are being applied to treat infections caused by Gram-negative bacteria, mainly in livestock, and are prescribed only in severe cases because of their adverse impacts on human health and the environment. Monitoring antibiotic residues in dairy products relies on the accessibility of portable and efficient analytical techniques. Presently, high-throughput screening techniques have been proposed to detect several antimicrobial drugs having identical structural and functional features. The L-histidine functionalized gold nanoparticles (His@AuNPs) do not form a complex with other tested antibiotic classes but show high selectivity for AMG antibiotics. We used ligand-induced aggregation of His@AuNPs as a rapid and sensitive localized surface plasmon resonance (LSPR) assay for AMG antibiotics, producing longitudinal extinction shifts at 660 nm. Herein, we explore the practical application of His@AuNPs to detect streptomycin spiked in water, milk, and whey fraction of milk with nanomolar level sensitivity. The ability of the analytical method to recognize target analytes sensitively and rapidly is of great significance to perform monitoring, thus would certainly reassure widespread use of AMG antibiotics. The biosynthesis of hybrid organic–inorganic metal nanoparticles like His@AuNPs with desired size distribution, stability, and specific host–guest recognition proficiency, would further facilitate applications in various other fields.
KW - Aminoglycoside
KW - Antibiotics
KW - Colorimetric changes
KW - Gold nanoparticles
KW - Histidine
KW - Milk samples
KW - Real samples
KW - Spectral shift
KW - Whey fraction
UR - http://www.scopus.com/inward/record.url?scp=85121820706&partnerID=8YFLogxK
U2 - 10.3390/chemosensors9120358
DO - 10.3390/chemosensors9120358
M3 - Article
AN - SCOPUS:85121820706
SN - 2227-9040
VL - 9
JO - Chemosensors
JF - Chemosensors
IS - 12
M1 - 358
ER -