Human papillomavirus type 16 E6 protein inhibits DNA fragmentation via interaction with DNA fragmentation factor 40

Jae Eun Jong; Kwi Wan Jeong; Hyokyung Shin; Lee Rang Hwang; Daeyoup Lee; Taegun Seo

doi:10.1016/j.canlet.2012.05.010

Human papillomavirus type 16 E6 protein inhibits DNA fragmentation via interaction with DNA fragmentation factor 40

Jae Eun Jong
, Kwi Wan Jeong
, Hyokyung Shin
, Lee Rang Hwang
, Daeyoup Lee
, Taegun Seo

Department of Life Science

Dongguk University
Gyeonggi Bio-Center
Korea Advanced Institute of Science and Technology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The E6 oncoprotein of human papillomavirus (HPV) is critical in cervical cancer development. Using the yeast two-hybrid assay, we showed that HPV-16 E6 (16E6) interacts with one of the DNA fragmentation factors (DFFs), DFF40, which mediates DNA degradation during apoptosis. Furthermore, 16E6 interacts with DFF40 through its zinc finger motif 2 and a bridge section linking the two zinc finger motifs. DNA fragmentation assays disclosed that 16E6 binding to DFF40 leads to blockage of DNA cleavage. Our data collectively suggest that suppression of DNA fragmentation through 16E6-DFF40 interaction is a central event promoting tumorigenesis.

Original language	English
Pages (from-to)	109-117
Number of pages	9
Journal	Cancer Letters
Volume	324
Issue number	1
DOIs	https://doi.org/10.1016/j.canlet.2012.05.010
State	Published - 1 Nov 2012

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

DNA fragmentation factor
E6 protein
Human papillomavirus-16

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10.1016/j.canlet.2012.05.010

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title = "Human papillomavirus type 16 E6 protein inhibits DNA fragmentation via interaction with DNA fragmentation factor 40",

abstract = "The E6 oncoprotein of human papillomavirus (HPV) is critical in cervical cancer development. Using the yeast two-hybrid assay, we showed that HPV-16 E6 (16E6) interacts with one of the DNA fragmentation factors (DFFs), DFF40, which mediates DNA degradation during apoptosis. Furthermore, 16E6 interacts with DFF40 through its zinc finger motif 2 and a bridge section linking the two zinc finger motifs. DNA fragmentation assays disclosed that 16E6 binding to DFF40 leads to blockage of DNA cleavage. Our data collectively suggest that suppression of DNA fragmentation through 16E6-DFF40 interaction is a central event promoting tumorigenesis.",

keywords = "DNA fragmentation factor, E6 protein, Human papillomavirus-16",

author = "Jong, \{Jae Eun\} and Jeong, \{Kwi Wan\} and Hyokyung Shin and Hwang, \{Lee Rang\} and Daeyoup Lee and Taegun Seo",

year = "2012",

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doi = "10.1016/j.canlet.2012.05.010",

language = "English",

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journal = "Cancer Letters",

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T1 - Human papillomavirus type 16 E6 protein inhibits DNA fragmentation via interaction with DNA fragmentation factor 40

AU - Jong, Jae Eun

AU - Jeong, Kwi Wan

AU - Shin, Hyokyung

AU - Hwang, Lee Rang

AU - Lee, Daeyoup

AU - Seo, Taegun

PY - 2012/11/1

Y1 - 2012/11/1

N2 - The E6 oncoprotein of human papillomavirus (HPV) is critical in cervical cancer development. Using the yeast two-hybrid assay, we showed that HPV-16 E6 (16E6) interacts with one of the DNA fragmentation factors (DFFs), DFF40, which mediates DNA degradation during apoptosis. Furthermore, 16E6 interacts with DFF40 through its zinc finger motif 2 and a bridge section linking the two zinc finger motifs. DNA fragmentation assays disclosed that 16E6 binding to DFF40 leads to blockage of DNA cleavage. Our data collectively suggest that suppression of DNA fragmentation through 16E6-DFF40 interaction is a central event promoting tumorigenesis.

AB - The E6 oncoprotein of human papillomavirus (HPV) is critical in cervical cancer development. Using the yeast two-hybrid assay, we showed that HPV-16 E6 (16E6) interacts with one of the DNA fragmentation factors (DFFs), DFF40, which mediates DNA degradation during apoptosis. Furthermore, 16E6 interacts with DFF40 through its zinc finger motif 2 and a bridge section linking the two zinc finger motifs. DNA fragmentation assays disclosed that 16E6 binding to DFF40 leads to blockage of DNA cleavage. Our data collectively suggest that suppression of DNA fragmentation through 16E6-DFF40 interaction is a central event promoting tumorigenesis.

KW - DNA fragmentation factor

KW - E6 protein

KW - Human papillomavirus-16

UR - https://www.scopus.com/pages/publications/84864059305

U2 - 10.1016/j.canlet.2012.05.010

DO - 10.1016/j.canlet.2012.05.010

M3 - Article

C2 - 22609799

AN - SCOPUS:84864059305

SN - 0304-3835

VL - 324

SP - 109

EP - 117

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

Human papillomavirus type 16 E6 protein inhibits DNA fragmentation via interaction with DNA fragmentation factor 40

Abstract

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Keywords

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