TY - JOUR
T1 - Human serum albumin-based propulsive Piperlongumine-loaded nanoparticles
T2 - Formulation development, characterization and anti-cancer study
AU - Patel, Krunal
AU - Jain, Poonam
AU - Rajput, Pradeep Kumar
AU - Jangid, Ashok Kumar
AU - Solanki, Raghu
AU - Kulhari, Hitesh
AU - Patel, Sunita
N1 - Publisher Copyright:
© 2022
PY - 2022/11/5
Y1 - 2022/11/5
N2 - Piperlongumine (PL) is a naturally occurring bioactive plant alkaloid, endowed with potential pharmacological activities, such as anti-inflammatory, antidiabetic, anti-atherosclerotic, anticancer and antibacterial properties. However, its hydrophobicity impose limitations in achieving great therapeutic efficacy. In the present study we have formulated Piperlongumine-loaded human serum albumin nanoparticles (PLNP) by desolvation method to enhance its bioavailability and anticancer potential. The resulting PLNPs were analyzed by Dynamic Light Scattering (DLS), High-resolution Transmission Electron Microscopy (HR-TEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The HR-TEM images confirmed the formation of spherical-shaped nanoparticles, with an average particle size of 105 nm. Further encapsulation of Piperlongumine in human serum albumin nanoparticle was confirmed by FTIR and the nature of encapsulation was characterized by XRD. PLNP showed a sustained drug release profile and higher anti-cancer efficacy against HCT 116 colorectal cancer cells. The PLNP synthesized were stable up to 3 months. The AO/EtBr staining, Hoechst 33342 assays, colony formation assay and mRNA expression studies revealed that the PLNP induced more apoptosis as compared to pure PL, in HCT 116 cells. Thus, PLNP can be used to enhance the therapeutic potential of pure Piperlongumine and a better therapeutic approach to treat colorectal cancer.
AB - Piperlongumine (PL) is a naturally occurring bioactive plant alkaloid, endowed with potential pharmacological activities, such as anti-inflammatory, antidiabetic, anti-atherosclerotic, anticancer and antibacterial properties. However, its hydrophobicity impose limitations in achieving great therapeutic efficacy. In the present study we have formulated Piperlongumine-loaded human serum albumin nanoparticles (PLNP) by desolvation method to enhance its bioavailability and anticancer potential. The resulting PLNPs were analyzed by Dynamic Light Scattering (DLS), High-resolution Transmission Electron Microscopy (HR-TEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The HR-TEM images confirmed the formation of spherical-shaped nanoparticles, with an average particle size of 105 nm. Further encapsulation of Piperlongumine in human serum albumin nanoparticle was confirmed by FTIR and the nature of encapsulation was characterized by XRD. PLNP showed a sustained drug release profile and higher anti-cancer efficacy against HCT 116 colorectal cancer cells. The PLNP synthesized were stable up to 3 months. The AO/EtBr staining, Hoechst 33342 assays, colony formation assay and mRNA expression studies revealed that the PLNP induced more apoptosis as compared to pure PL, in HCT 116 cells. Thus, PLNP can be used to enhance the therapeutic potential of pure Piperlongumine and a better therapeutic approach to treat colorectal cancer.
KW - Colon cancer
KW - Drug delivery
KW - Human Serum Albumin nanoparticle
KW - Piperlongumine
UR - http://www.scopus.com/inward/record.url?scp=85135310849&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfa.2022.129738
DO - 10.1016/j.colsurfa.2022.129738
M3 - Article
AN - SCOPUS:85135310849
SN - 0927-7757
VL - 652
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects
M1 - 129738
ER -
