Hybrid microarray based on double biomolecular markers of DNA and carbohydrate for simultaneous genotypic and phenotypic detection of cholera toxin-producing Vibrio cholerae

Hwa Hui Shin, Jeong Hyun Seo, Chang Sup Kim, Byeong Hee Hwang, Hyung Joon Cha

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Life-threatening diarrheal cholera is usually caused by water or food contaminated with cholera toxin-producing Vibrio cholerae. For the prevention and surveillance of cholera, it is crucial to rapidly and precisely detect and identify the etiological causes, such as V. cholerae and/or its toxin. In the present work, we propose the use of a hybrid double biomolecular marker (DBM) microarray containing 16S rRNA-based DNA capture probe to genotypically identify V. cholerae and GM1 pentasaccharide capture probe to phenotypically detect cholera toxin. We employed a simple sample preparation method to directly obtain genomic DNA and secreted cholera toxin as target materials from bacterial cells. By utilizing the constructed DBM microarray and prepared samples, V. cholerae and cholera toxin were detected successfully, selectively, and simultaneously; the DBM microarray was able to analyze the pathogenicity of the identified V. cholerae regardless of whether the bacteria produces toxin. Therefore, our proposed DBM microarray is a new effective platform for identifying bacteria and analyzing bacterial pathogenicity simultaneously.

Original languageEnglish
Pages (from-to)398-405
Number of pages8
JournalBiosensors and Bioelectronics
Volume79
DOIs
StatePublished - 15 May 2016

Keywords

  • Carbohydrate probe
  • Cholera toxin
  • DNA probe
  • Double biomolecular markers
  • Pathogenicity
  • Vibrio cholerae

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