Abstract
Premise of the study: Positional cloning in combination with phenotyping is a general approach to identify disease-resistance gene candidates in plants; however, it requires several time-consuming steps including population or fine mapping. Therefore, in the present study, we suggest a new combined strategy to improve the identification of disease-resistance gene candidates. Methods and Results: Downy mildew (DM)-resistant maize was selected from five cultivars using a spreader row technique. Positional cloning and bioinformatics tools were used to identify the DM-resistance quantitative trait locus marker (bnlg1702) and 47 protein-coding gene annotations. Eventually, five DM-resistance gene candidates, including bZIP34, Bak1, and Ppr, were identified by quantitative reverse-transcription PCR (RT-PCR) without fine mapping of the bnlg1702 locus. Conclusions: The combined protocol with the spreader row technique, quantitative trait locus positional cloning, and quantitative RT-PCR was effective for identifying DM-resistance candidate genes. This cloning approach may be applied to other wholegenome-sequenced crops or resistance to other diseases.
Original language | English |
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Article number | 1600132 |
Journal | Applications in Plant Sciences |
Volume | 5 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2017 |
Keywords
- downy mildew
- maize
- positional cloning
- quantitative reverse-transcription PCR (RT-PCR)
- spreader row technique