Immobilization and characterization of ferritin on gold electrode

Chul Wan Park, Hyun Ju Park, Ji Hyeon Kim, Keehoon Won, Hyon Hee Yoon

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Properties of ferritin, immobilized on dithiobis (N-succinimydyl propionate) (DTSP)-covered gold electrode, in 3-morpholino propanesulfonic acid buffer were investigated by AFM and FE-SEM. Electrochemical properties the ferritin was measured by a cyclic volatammetry. When the potentials of 0.2, 0.34, and 1.0 V vs. Ag/AgCl were applied for 15 h for the ferritin immobilization, the electrode potential of Fe(II)/Fe(III) in ferritin changed to negative values. Negative electrode potential shift of Fe(II)/Fe(III) in ferritin with respect to the applied potential could be attributed to the stabilized ferritin on DTSP-covered gold electrode. From AFM and SEM images, it was proven that ferritins fixed at below 0.34 V were clusters in several micrometer and those fixed at higher applied potential than OCV were finely distributed particles in several tens of nanometer.

Original languageEnglish
Pages (from-to)1001-1005
Number of pages5
JournalUltramicroscopy
Volume109
Issue number8
DOIs
StatePublished - Jul 2009

Keywords

  • Atomic force microscope
  • Cyclic voltammogram
  • Ferritin immobilization
  • Gold electrode

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