Abstract
Properties of ferritin, immobilized on dithiobis (N-succinimydyl propionate) (DTSP)-covered gold electrode, in 3-morpholino propanesulfonic acid buffer were investigated by AFM and FE-SEM. Electrochemical properties the ferritin was measured by a cyclic volatammetry. When the potentials of 0.2, 0.34, and 1.0 V vs. Ag/AgCl were applied for 15 h for the ferritin immobilization, the electrode potential of Fe(II)/Fe(III) in ferritin changed to negative values. Negative electrode potential shift of Fe(II)/Fe(III) in ferritin with respect to the applied potential could be attributed to the stabilized ferritin on DTSP-covered gold electrode. From AFM and SEM images, it was proven that ferritins fixed at below 0.34 V were clusters in several micrometer and those fixed at higher applied potential than OCV were finely distributed particles in several tens of nanometer.
Original language | English |
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Pages (from-to) | 1001-1005 |
Number of pages | 5 |
Journal | Ultramicroscopy |
Volume | 109 |
Issue number | 8 |
DOIs | |
State | Published - Jul 2009 |
Keywords
- Atomic force microscope
- Cyclic voltammogram
- Ferritin immobilization
- Gold electrode