Immobilization and characterization of ferritin on gold electrode

Chul Wan Park; Hyun Ju Park; Ji Hyeon Kim; Keehoon Won; Hyon Hee Yoon

doi:10.1016/j.ultramic.2009.03.002

Immobilization and characterization of ferritin on gold electrode

Chul Wan Park
, Hyun Ju Park
, Ji Hyeon Kim
, Keehoon Won
, Hyon Hee Yoon

Department of Chemical and Biochemical Engineering

Gachon University

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Properties of ferritin, immobilized on dithiobis (N-succinimydyl propionate) (DTSP)-covered gold electrode, in 3-morpholino propanesulfonic acid buffer were investigated by AFM and FE-SEM. Electrochemical properties the ferritin was measured by a cyclic volatammetry. When the potentials of 0.2, 0.34, and 1.0 V vs. Ag/AgCl were applied for 15 h for the ferritin immobilization, the electrode potential of Fe(II)/Fe(III) in ferritin changed to negative values. Negative electrode potential shift of Fe(II)/Fe(III) in ferritin with respect to the applied potential could be attributed to the stabilized ferritin on DTSP-covered gold electrode. From AFM and SEM images, it was proven that ferritins fixed at below 0.34 V were clusters in several micrometer and those fixed at higher applied potential than OCV were finely distributed particles in several tens of nanometer.

Original language	English
Pages (from-to)	1001-1005
Number of pages	5
Journal	Ultramicroscopy
Volume	109
Issue number	8
DOIs	https://doi.org/10.1016/j.ultramic.2009.03.002
State	Published - Jul 2009

Keywords

Atomic force microscope
Cyclic voltammogram
Ferritin immobilization
Gold electrode

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10.1016/j.ultramic.2009.03.002

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title = "Immobilization and characterization of ferritin on gold electrode",

abstract = "Properties of ferritin, immobilized on dithiobis (N-succinimydyl propionate) (DTSP)-covered gold electrode, in 3-morpholino propanesulfonic acid buffer were investigated by AFM and FE-SEM. Electrochemical properties the ferritin was measured by a cyclic volatammetry. When the potentials of 0.2, 0.34, and 1.0 V vs. Ag/AgCl were applied for 15 h for the ferritin immobilization, the electrode potential of Fe(II)/Fe(III) in ferritin changed to negative values. Negative electrode potential shift of Fe(II)/Fe(III) in ferritin with respect to the applied potential could be attributed to the stabilized ferritin on DTSP-covered gold electrode. From AFM and SEM images, it was proven that ferritins fixed at below 0.34 V were clusters in several micrometer and those fixed at higher applied potential than OCV were finely distributed particles in several tens of nanometer.",

keywords = "Atomic force microscope, Cyclic voltammogram, Ferritin immobilization, Gold electrode",

author = "Park, \{Chul Wan\} and Park, \{Hyun Ju\} and Kim, \{Ji Hyeon\} and Keehoon Won and Yoon, \{Hyon Hee\}",

year = "2009",

month = jul,

doi = "10.1016/j.ultramic.2009.03.002",

language = "English",

volume = "109",

pages = "1001--1005",

journal = "Ultramicroscopy",

issn = "0304-3991",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Immobilization and characterization of ferritin on gold electrode

AU - Park, Chul Wan

AU - Park, Hyun Ju

AU - Kim, Ji Hyeon

AU - Won, Keehoon

AU - Yoon, Hyon Hee

PY - 2009/7

Y1 - 2009/7

N2 - Properties of ferritin, immobilized on dithiobis (N-succinimydyl propionate) (DTSP)-covered gold electrode, in 3-morpholino propanesulfonic acid buffer were investigated by AFM and FE-SEM. Electrochemical properties the ferritin was measured by a cyclic volatammetry. When the potentials of 0.2, 0.34, and 1.0 V vs. Ag/AgCl were applied for 15 h for the ferritin immobilization, the electrode potential of Fe(II)/Fe(III) in ferritin changed to negative values. Negative electrode potential shift of Fe(II)/Fe(III) in ferritin with respect to the applied potential could be attributed to the stabilized ferritin on DTSP-covered gold electrode. From AFM and SEM images, it was proven that ferritins fixed at below 0.34 V were clusters in several micrometer and those fixed at higher applied potential than OCV were finely distributed particles in several tens of nanometer.

AB - Properties of ferritin, immobilized on dithiobis (N-succinimydyl propionate) (DTSP)-covered gold electrode, in 3-morpholino propanesulfonic acid buffer were investigated by AFM and FE-SEM. Electrochemical properties the ferritin was measured by a cyclic volatammetry. When the potentials of 0.2, 0.34, and 1.0 V vs. Ag/AgCl were applied for 15 h for the ferritin immobilization, the electrode potential of Fe(II)/Fe(III) in ferritin changed to negative values. Negative electrode potential shift of Fe(II)/Fe(III) in ferritin with respect to the applied potential could be attributed to the stabilized ferritin on DTSP-covered gold electrode. From AFM and SEM images, it was proven that ferritins fixed at below 0.34 V were clusters in several micrometer and those fixed at higher applied potential than OCV were finely distributed particles in several tens of nanometer.

KW - Atomic force microscope

KW - Cyclic voltammogram

KW - Ferritin immobilization

KW - Gold electrode

UR - https://www.scopus.com/pages/publications/67449161572

U2 - 10.1016/j.ultramic.2009.03.002

DO - 10.1016/j.ultramic.2009.03.002

M3 - Article

AN - SCOPUS:67449161572

SN - 0304-3991

VL - 109

SP - 1001

EP - 1005

JO - Ultramicroscopy

JF - Ultramicroscopy

IS - 8

ER -

Immobilization and characterization of ferritin on gold electrode

Abstract

Keywords

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