Immobilization method of escherichia coli for microfluidic application

I. Kim, T. An, W. Choi, C. S. Kim, H. J. Cha, G. Lim

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Escherichia coli (E. coli) has been used extensively in various industrial and scientific applications but has not been used widely for microfluidic applications because it is difficult to immobilize properly to a device. Here, we describe the development of an E. coli immobilization method for microfluidic devices using single-walled nanotubes (SWNTs) and dielectrophoresis (DEP) force. SWNTs and E. coli were aligned between two cantilever electrodes by a positive DEP force, forming a film of SWNTs with attached E. coli. For a microfluidic device, our one-step immobilization method has many advantages such as site-specific immobilization, easy density and shape control, an electrically connected structure from the electrodes to the E. coli, and rapid processing.

Original languageEnglish
Title of host publication2013 Transducers and Eurosensors XXVII
Subtitle of host publicationThe 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Pages598-601
Number of pages4
DOIs
StatePublished - 2013
Event2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 - Barcelona, Spain
Duration: 16 Jun 201320 Jun 2013

Publication series

Name2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

Conference

Conference2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Country/TerritorySpain
CityBarcelona
Period16/06/1320/06/13

Keywords

  • carbon nanotubes
  • dielectrophoresis
  • Escherichia coli
  • microbial immobilization

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