Immunosuppressive property of dried human amniotic membrane

Choul Yong Park; Sahar Kohanim; Lei Zhu; Peter L. Gehlbach; Roy S. Chuck

doi:10.1159/000187629

Immunosuppressive property of dried human amniotic membrane

Choul Yong Park
, Sahar Kohanim
, Lei Zhu
, Peter L. Gehlbach
, Roy S. Chuck

Department of Medicine

Johns Hopkins University

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Purpose: To report the immunosuppressive property of dry human amniotic membrane (dHAM). Methods: Mouse splenocytes harvested from Balb/c mice were stimulated using functional-grade anti-CD3e antibodies for 4 days either with or without either of 2 commercial types of dHAM (Ambiodry 1 & 2, IOP Inc., Costa Mesa, Calif., USA) added to the culture media. The cell proliferation assay was performed to analyze the extent of splenocyte proliferation. Results: dHAMs significantly suppressed mouse splenocyte proliferation compared to control. The suppression by dHAM with intact amniotic epithelium (Ambiodry 2) was significantly stronger than dHAM without epithelium (Ambiodry 1). Conclusion: The immunosuppressive property of dHAM was demonstrated using an alloreactive splenocyte proliferation assay.

Original language	English
Pages (from-to)	112-113
Number of pages	2
Journal	Ophthalmic Research
Volume	41
Issue number	2
DOIs	https://doi.org/10.1159/000187629
State	Published - Jan 2009

Keywords

Amniotic membrane
Cryopreservation
Immune suppression
Ocular surface
T cell proliferation

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10.1159/000187629

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title = "Immunosuppressive property of dried human amniotic membrane",

abstract = "Purpose: To report the immunosuppressive property of dry human amniotic membrane (dHAM). Methods: Mouse splenocytes harvested from Balb/c mice were stimulated using functional-grade anti-CD3e antibodies for 4 days either with or without either of 2 commercial types of dHAM (Ambiodry 1 \& 2, IOP Inc., Costa Mesa, Calif., USA) added to the culture media. The cell proliferation assay was performed to analyze the extent of splenocyte proliferation. Results: dHAMs significantly suppressed mouse splenocyte proliferation compared to control. The suppression by dHAM with intact amniotic epithelium (Ambiodry 2) was significantly stronger than dHAM without epithelium (Ambiodry 1). Conclusion: The immunosuppressive property of dHAM was demonstrated using an alloreactive splenocyte proliferation assay.",

keywords = "Amniotic membrane, Cryopreservation, Immune suppression, Ocular surface, T cell proliferation",

author = "Park, \{Choul Yong\} and Sahar Kohanim and Lei Zhu and Gehlbach, \{Peter L.\} and Chuck, \{Roy S.\}",

year = "2009",

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doi = "10.1159/000187629",

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TY - JOUR

T1 - Immunosuppressive property of dried human amniotic membrane

AU - Park, Choul Yong

AU - Kohanim, Sahar

AU - Zhu, Lei

AU - Gehlbach, Peter L.

AU - Chuck, Roy S.

PY - 2009/1

Y1 - 2009/1

N2 - Purpose: To report the immunosuppressive property of dry human amniotic membrane (dHAM). Methods: Mouse splenocytes harvested from Balb/c mice were stimulated using functional-grade anti-CD3e antibodies for 4 days either with or without either of 2 commercial types of dHAM (Ambiodry 1 & 2, IOP Inc., Costa Mesa, Calif., USA) added to the culture media. The cell proliferation assay was performed to analyze the extent of splenocyte proliferation. Results: dHAMs significantly suppressed mouse splenocyte proliferation compared to control. The suppression by dHAM with intact amniotic epithelium (Ambiodry 2) was significantly stronger than dHAM without epithelium (Ambiodry 1). Conclusion: The immunosuppressive property of dHAM was demonstrated using an alloreactive splenocyte proliferation assay.

AB - Purpose: To report the immunosuppressive property of dry human amniotic membrane (dHAM). Methods: Mouse splenocytes harvested from Balb/c mice were stimulated using functional-grade anti-CD3e antibodies for 4 days either with or without either of 2 commercial types of dHAM (Ambiodry 1 & 2, IOP Inc., Costa Mesa, Calif., USA) added to the culture media. The cell proliferation assay was performed to analyze the extent of splenocyte proliferation. Results: dHAMs significantly suppressed mouse splenocyte proliferation compared to control. The suppression by dHAM with intact amniotic epithelium (Ambiodry 2) was significantly stronger than dHAM without epithelium (Ambiodry 1). Conclusion: The immunosuppressive property of dHAM was demonstrated using an alloreactive splenocyte proliferation assay.

KW - Amniotic membrane

KW - Cryopreservation

KW - Immune suppression

KW - Ocular surface

KW - T cell proliferation

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DO - 10.1159/000187629

M3 - Article

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SN - 0030-3747

VL - 41

SP - 112

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JO - Ophthalmic Research

JF - Ophthalmic Research

IS - 2

ER -

Immunosuppressive property of dried human amniotic membrane

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