TY - JOUR
T1 - Induction of ICAM-1 by Armillariella mellea is mediated through generation of reactive oxygen species and JNK activation
AU - Kim, Young Sook
AU - Im, Jintaek
AU - Choi, Jung Nam
AU - Kang, Seok Seong
AU - Lee, Yeo Jin
AU - Lee, Choong Hwan
AU - Yun, Cheol Heui
AU - Son, Chang Gue
AU - Han, Seung Hyun
PY - 2010/3
Y1 - 2010/3
N2 - Ethnopharmacological relevance: Armillariella mellea is an edible mushroom that has been traditionally used as an alternative medicine in many countries because of its anti-microbial and anti-cancer effects. Aim of the study: In this study, we examined the ability of Armillariella mellea to induce the expression of intercellular adhesion molecule (ICAM)-1, an important cellular adhesion molecule for the recruitment of immune cells to regional inflammatory sites. Materials and methods: A human monocytic cell line, THP-1 or human peripheral blood mononuclear cells (PBMC) were stimulated with Armillariella mellea extract (AME) and subjected to flow cytometry to examine the expression of ICAM-1 protein on the cell surface. Steady-state mRNA level of ICAM-1 was determined by real-time reverse transcription-polymerase chain reaction. The phosphorylation of JNK protein was examined by Western blot analysis using antibodies specific for non-phosphorylated and phosphorylated forms of JNK. For the analysis of transcription factors regulating ICAM-1 transcription, the nuclear fraction was extracted from AME-treated THP-1 cells and subjected to electrophoretic mobility shift assay. Results: AME induced expression of ICAM-1 and its mRNA in THP-1 cells in dose- and time-dependent manners. AME-induced ICAM-1 expression was also observed on CD14-positive monocytes in human PBMC. Interestingly, AME-induced ICAM-1 production was inhibited by the specific inhibitors of reactive oxygen species (ROS) and JNK, whereas no inhibitory effect was observed when inhibitors of ERK, p38 kinase, phosphatidylinositol 3-kinase, or protein kinase C were used. Concomitantly, AME increased phosphorylation of JNK in a time-dependent fashion. DNA binding activities of NF-κB, AP-1, SP-1, and STAT-1 were increased by AME treatment. Conclusion: These results suggest that AME induces ICAM-1 expression in human monocytic cells through ROS/JNK-dependent signaling pathways leading to the activation of NF-κB, AP-1, SP-1, and STAT-1 transcription factors.
AB - Ethnopharmacological relevance: Armillariella mellea is an edible mushroom that has been traditionally used as an alternative medicine in many countries because of its anti-microbial and anti-cancer effects. Aim of the study: In this study, we examined the ability of Armillariella mellea to induce the expression of intercellular adhesion molecule (ICAM)-1, an important cellular adhesion molecule for the recruitment of immune cells to regional inflammatory sites. Materials and methods: A human monocytic cell line, THP-1 or human peripheral blood mononuclear cells (PBMC) were stimulated with Armillariella mellea extract (AME) and subjected to flow cytometry to examine the expression of ICAM-1 protein on the cell surface. Steady-state mRNA level of ICAM-1 was determined by real-time reverse transcription-polymerase chain reaction. The phosphorylation of JNK protein was examined by Western blot analysis using antibodies specific for non-phosphorylated and phosphorylated forms of JNK. For the analysis of transcription factors regulating ICAM-1 transcription, the nuclear fraction was extracted from AME-treated THP-1 cells and subjected to electrophoretic mobility shift assay. Results: AME induced expression of ICAM-1 and its mRNA in THP-1 cells in dose- and time-dependent manners. AME-induced ICAM-1 expression was also observed on CD14-positive monocytes in human PBMC. Interestingly, AME-induced ICAM-1 production was inhibited by the specific inhibitors of reactive oxygen species (ROS) and JNK, whereas no inhibitory effect was observed when inhibitors of ERK, p38 kinase, phosphatidylinositol 3-kinase, or protein kinase C were used. Concomitantly, AME increased phosphorylation of JNK in a time-dependent fashion. DNA binding activities of NF-κB, AP-1, SP-1, and STAT-1 were increased by AME treatment. Conclusion: These results suggest that AME induces ICAM-1 expression in human monocytic cells through ROS/JNK-dependent signaling pathways leading to the activation of NF-κB, AP-1, SP-1, and STAT-1 transcription factors.
KW - Armillariella mellea
KW - Immuno-regulation
KW - Intercellular adhesion molecule-1
KW - MAP kinase
KW - Medicinal mushroom
KW - Reactive oxygen species
KW - Transcription factors
UR - http://www.scopus.com/inward/record.url?scp=76749097553&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2010.01.011
DO - 10.1016/j.jep.2010.01.011
M3 - Article
C2 - 20079413
AN - SCOPUS:76749097553
SN - 0378-8741
VL - 128
SP - 198
EP - 205
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
IS - 1
ER -