Influence of PEG chain length on colloidal stability of mPEGylated polycation based coacersomes for therapeutic protein delivery

Heejung Jo, Mani Gajendiran, Kyobum Kim

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The polycation/polyanion based coacervate platform for therapeutic protein delivery finds difficulty in their colloidal stability under physiological environment. To overcome this issue, PEGylation could be a versatile strategy to enhance colloidal stability. Herein, a poly(ethylene arginyl aspartate diglyceride) (PEAD) polycation was synthesized, and a series of methoxy polyethylene glycols (mPEG) including mPEG350, mPEG750 and mPEG2000 were attached with PEAD polycation to obtain mPEG350-PEAD, mPEG750-PEAD and mPEG2000-PEAD respectively. The PEAD and mPEGylated PEADs were complexed with heparin (HEP) to fabricate coacervate (Coa) and coacersome (mP_Coa) particles respectively. The colloidal stability of Coa and mP_Coa coacersomes has been investigated by dynamic light scattering (DLS) and microscopic techniques. The vascular endothelial growth factor 165 (VEGF-165) was encapsulated in the Coa or mP_Coa particles and administered to human umbilical vein endothelial cells (HUVECs) to induce a tubular network formation in vitro. All the polycations are highly biocompatible and exhibit more than 94 % of VEGF-165 loading efficiency. An effect of mPEG chain length on colloidal stability of mP_Coa and in vitro tubular formation ability of HUVECs has been investigated.

Original languageEnglish
Pages (from-to)234-242
Number of pages9
JournalJournal of Industrial and Engineering Chemistry
Volume82
DOIs
StatePublished - 25 Feb 2020

Keywords

  • Coacersome
  • Colloidal stability
  • mPEGylation
  • Polycation
  • Protein delivery

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