Inhibitory effect of Salvia plebeia leaf extract on ultraviolet-induced photoaging-associated ion channels and enzymes

You Jin Chang, Dong Ung Lee, Da Yeong Nam, Sung Min Cho, Seungug Hong, Joo Hyun Nam, Woo Kyung Kim

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

In traditional Korean/Asian medicine, Salvia plebeia R.Br. (S. plebeia) leaves are used to treat inflammatory diseases, including dermatitis, cough, asthma and toothache. Recently, S. plebeia leaves have been applied in skin care, as they promote skin lightening and elasticity. Therefore, the present study investigated the anti aging effects of S. plebeia leaf methanolic extract and its fractions (dichloromethane, ethylacetate and n butanol). The results of a whole cell patch clamp analysis indicated that the methanolic extract mediated ultraviolet (UV) induced photoaging associated ion channels, transient receptor potential vanilloid 1 (TRPV1) and calcium release activated calcium channel protein 1 (ORAI1) channel activity in HEK293T cells overexpressing TRPV1 or ORAI1 and STIM1. Electrophysiological analysis revealed that the butanol fraction inhibited capsaicin induced TRPV1 (84±8% at-60 mV/86±1% at 100 mV at 100 μg/ml) and ORAI1 (87±2% at-120 mV at 100 μg/ml) currents. Furthermore, the dichloromethane and hexane fractions inhibited tyrosinase activity by 32.4±0.69 and 22.6±0.96% at 330 μg/ml, respectively. Furthermore, the ethylacetate and butanol fractions inhibited elastase activity by 65.2±1.30 and 31.7±1.23% at 330 μg/ml, respectively. Tyrosinase and elastase, which are UV induced photoaging associated enzymes, regulate skin pigmentation and wrinkle formation, respectively. The results of the present study indicated that S. plebeia leaves may be a novel treatment for UV induced photoaging.

Original languageEnglish
Pages (from-to)567-575
Number of pages9
JournalExperimental and Therapeutic Medicine
Volume13
Issue number2
DOIs
StatePublished - Feb 2017

Keywords

  • Calcium channel
  • Calcium release activated calcium channel protein 1
  • Elastase activity
  • Salvia plebeia
  • Transient receptor potential vanilloid 1
  • Tyrosinase activity
  • Ultraviolet induced photoaging

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