TY - JOUR
T1 - Inhibitory effect of Salvia plebeia leaf extract on ultraviolet-induced photoaging-associated ion channels and enzymes
AU - Chang, You Jin
AU - Lee, Dong Ung
AU - Nam, Da Yeong
AU - Cho, Sung Min
AU - Hong, Seungug
AU - Nam, Joo Hyun
AU - Kim, Woo Kyung
N1 - Publisher Copyright:
© 2016, Spandidos Publications. All rights reserved.
PY - 2017/2
Y1 - 2017/2
N2 - In traditional Korean/Asian medicine, Salvia plebeia R.Br. (S. plebeia) leaves are used to treat inflammatory diseases, including dermatitis, cough, asthma and toothache. Recently, S. plebeia leaves have been applied in skin care, as they promote skin lightening and elasticity. Therefore, the present study investigated the anti aging effects of S. plebeia leaf methanolic extract and its fractions (dichloromethane, ethylacetate and n butanol). The results of a whole cell patch clamp analysis indicated that the methanolic extract mediated ultraviolet (UV) induced photoaging associated ion channels, transient receptor potential vanilloid 1 (TRPV1) and calcium release activated calcium channel protein 1 (ORAI1) channel activity in HEK293T cells overexpressing TRPV1 or ORAI1 and STIM1. Electrophysiological analysis revealed that the butanol fraction inhibited capsaicin induced TRPV1 (84±8% at-60 mV/86±1% at 100 mV at 100 μg/ml) and ORAI1 (87±2% at-120 mV at 100 μg/ml) currents. Furthermore, the dichloromethane and hexane fractions inhibited tyrosinase activity by 32.4±0.69 and 22.6±0.96% at 330 μg/ml, respectively. Furthermore, the ethylacetate and butanol fractions inhibited elastase activity by 65.2±1.30 and 31.7±1.23% at 330 μg/ml, respectively. Tyrosinase and elastase, which are UV induced photoaging associated enzymes, regulate skin pigmentation and wrinkle formation, respectively. The results of the present study indicated that S. plebeia leaves may be a novel treatment for UV induced photoaging.
AB - In traditional Korean/Asian medicine, Salvia plebeia R.Br. (S. plebeia) leaves are used to treat inflammatory diseases, including dermatitis, cough, asthma and toothache. Recently, S. plebeia leaves have been applied in skin care, as they promote skin lightening and elasticity. Therefore, the present study investigated the anti aging effects of S. plebeia leaf methanolic extract and its fractions (dichloromethane, ethylacetate and n butanol). The results of a whole cell patch clamp analysis indicated that the methanolic extract mediated ultraviolet (UV) induced photoaging associated ion channels, transient receptor potential vanilloid 1 (TRPV1) and calcium release activated calcium channel protein 1 (ORAI1) channel activity in HEK293T cells overexpressing TRPV1 or ORAI1 and STIM1. Electrophysiological analysis revealed that the butanol fraction inhibited capsaicin induced TRPV1 (84±8% at-60 mV/86±1% at 100 mV at 100 μg/ml) and ORAI1 (87±2% at-120 mV at 100 μg/ml) currents. Furthermore, the dichloromethane and hexane fractions inhibited tyrosinase activity by 32.4±0.69 and 22.6±0.96% at 330 μg/ml, respectively. Furthermore, the ethylacetate and butanol fractions inhibited elastase activity by 65.2±1.30 and 31.7±1.23% at 330 μg/ml, respectively. Tyrosinase and elastase, which are UV induced photoaging associated enzymes, regulate skin pigmentation and wrinkle formation, respectively. The results of the present study indicated that S. plebeia leaves may be a novel treatment for UV induced photoaging.
KW - Calcium channel
KW - Calcium release activated calcium channel protein 1
KW - Elastase activity
KW - Salvia plebeia
KW - Transient receptor potential vanilloid 1
KW - Tyrosinase activity
KW - Ultraviolet induced photoaging
UR - http://www.scopus.com/inward/record.url?scp=85009423410&partnerID=8YFLogxK
U2 - 10.3892/etm.2017.4025
DO - 10.3892/etm.2017.4025
M3 - Article
AN - SCOPUS:85009423410
SN - 1792-0981
VL - 13
SP - 567
EP - 575
JO - Experimental and Therapeutic Medicine
JF - Experimental and Therapeutic Medicine
IS - 2
ER -