Intein-mediated protein engineering for biosensor fabrication

Hyunjin Jeon, Minhyeong Lee, Wonhee Jang, Youngeun Kwon

Research output: Contribution to journalReview articlepeer-review

9 Scopus citations

Abstract

Intein-mediated protein engineering has become a valuable tool for biosensing applications. Expressed protein ligation and protein trans-splicing were used to generate proteins with necessary tags or cyclic peptides that can be used to build various biosensing platforms such as biochips or sensor proteins. Especially the use of split-inteins that carry out conditional protein splicing reaction enabled the fabrication of various genetically-encoded biosensors that can monitor various signaling events in vivo including protein- protein interactions and protein translocalization. Biological targets activated split-inteins to generate functioning reporter molecules, such as luciferase and autofluorescent proteins. We here review various sensing platforms that utilize intein-mediated protein engineering technology mainly focusing on cell-based biosensors.

Original languageEnglish
Pages (from-to)277-287
Number of pages11
JournalBiochip Journal
Volume10
Issue number4
DOIs
StatePublished - 1 Dec 2016

Keywords

  • Biosensors
  • Cell-based sensors
  • Inteins
  • Protein engineering
  • Protein trans-splicing

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