Intrinsic toxicity of stable nanosized titanium dioxide using polyacrylate in human keratinocytes

Preeyaporn Koedrith; Yeo Jin Kim; Younghun Kim; Joo Hyon Kang; Young Rok Seo

doi:10.1007/s13273-018-0030-4

Intrinsic toxicity of stable nanosized titanium dioxide using polyacrylate in human keratinocytes

Preeyaporn Koedrith, Yeo Jin Kim, Younghun Kim, Joo Hyon Kang, Young Rok Seo

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Backgrounds: Trends in the use of an anticoagulant as a dispersing stabilizer are addressed. An effective approach to preparing stable nanosized titanium dioxide (nTiO ₂ ) for accurate and systematic assessment of nano- toxicity has not been established. Methods: Among the dispersants tested here, it was found that sodium polyacrylate (PAA) was the most effective dispersant for nTiO ₂ in culture media. Our study was the first to demonstrate that a stable PAA-dispersed nTiO ₂ (nTiO ₂ /PAA) suspension showed more toxic than nTiO ₂ without PAA in human HaCaT keratinocytes. Results: Initially, MTT results showed that the stable nTiO ₂ /PAA dispersion exhibited significantly greater cytotoxicity than nTiO ₂ without PAA. In addition, the stable nTiO ₂ /PAA dispersion induced markedly more oxidative stress than nTiO ₂ without PAA. Importantly, the stable nTiO ₂ /PAA dispersion caused DNA breakage to a greater extent than nTiO ₂ without PAA. Conclusion: Our findings indicated that the anti-coagulant PAA is suitable for preparing homologous dispersed nTiO ₂ under realistic physiological culture test conditions.

Original language	English
Pages (from-to)	273-282
Number of pages	10
Journal	Molecular and Cellular Toxicology
Volume	14
Issue number	3
DOIs	https://doi.org/10.1007/s13273-018-0030-4
State	Published - 1 Jul 2018

Keywords

Cytotoxicity
Dispersing stabilizer
Genotoxicity
Human keratinocytes
Titanium dioxide nanoparticles

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10.1007/s13273-018-0030-4

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title = "Intrinsic toxicity of stable nanosized titanium dioxide using polyacrylate in human keratinocytes",

abstract = " Backgrounds: Trends in the use of an anticoagulant as a dispersing stabilizer are addressed. An effective approach to preparing stable nanosized titanium dioxide (nTiO 2 ) for accurate and systematic assessment of nano- toxicity has not been established. Methods: Among the dispersants tested here, it was found that sodium polyacrylate (PAA) was the most effective dispersant for nTiO 2 in culture media. Our study was the first to demonstrate that a stable PAA-dispersed nTiO 2 (nTiO 2 /PAA) suspension showed more toxic than nTiO 2 without PAA in human HaCaT keratinocytes. Results: Initially, MTT results showed that the stable nTiO 2 /PAA dispersion exhibited significantly greater cytotoxicity than nTiO 2 without PAA. In addition, the stable nTiO 2 /PAA dispersion induced markedly more oxidative stress than nTiO 2 without PAA. Importantly, the stable nTiO 2 /PAA dispersion caused DNA breakage to a greater extent than nTiO 2 without PAA. Conclusion: Our findings indicated that the anti-coagulant PAA is suitable for preparing homologous dispersed nTiO 2 under realistic physiological culture test conditions.",

keywords = "Cytotoxicity, Dispersing stabilizer, Genotoxicity, Human keratinocytes, Titanium dioxide nanoparticles",

author = "Preeyaporn Koedrith and Kim, {Yeo Jin} and Younghun Kim and Kang, {Joo Hyon} and Seo, {Young Rok}",

note = "Publisher Copyright: {\textcopyright} 2018, The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Nature B.V.",

year = "2018",

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doi = "10.1007/s13273-018-0030-4",

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T1 - Intrinsic toxicity of stable nanosized titanium dioxide using polyacrylate in human keratinocytes

AU - Koedrith, Preeyaporn

AU - Kim, Yeo Jin

AU - Kim, Younghun

AU - Kang, Joo Hyon

AU - Seo, Young Rok

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Backgrounds: Trends in the use of an anticoagulant as a dispersing stabilizer are addressed. An effective approach to preparing stable nanosized titanium dioxide (nTiO 2 ) for accurate and systematic assessment of nano- toxicity has not been established. Methods: Among the dispersants tested here, it was found that sodium polyacrylate (PAA) was the most effective dispersant for nTiO 2 in culture media. Our study was the first to demonstrate that a stable PAA-dispersed nTiO 2 (nTiO 2 /PAA) suspension showed more toxic than nTiO 2 without PAA in human HaCaT keratinocytes. Results: Initially, MTT results showed that the stable nTiO 2 /PAA dispersion exhibited significantly greater cytotoxicity than nTiO 2 without PAA. In addition, the stable nTiO 2 /PAA dispersion induced markedly more oxidative stress than nTiO 2 without PAA. Importantly, the stable nTiO 2 /PAA dispersion caused DNA breakage to a greater extent than nTiO 2 without PAA. Conclusion: Our findings indicated that the anti-coagulant PAA is suitable for preparing homologous dispersed nTiO 2 under realistic physiological culture test conditions.

AB - Backgrounds: Trends in the use of an anticoagulant as a dispersing stabilizer are addressed. An effective approach to preparing stable nanosized titanium dioxide (nTiO 2 ) for accurate and systematic assessment of nano- toxicity has not been established. Methods: Among the dispersants tested here, it was found that sodium polyacrylate (PAA) was the most effective dispersant for nTiO 2 in culture media. Our study was the first to demonstrate that a stable PAA-dispersed nTiO 2 (nTiO 2 /PAA) suspension showed more toxic than nTiO 2 without PAA in human HaCaT keratinocytes. Results: Initially, MTT results showed that the stable nTiO 2 /PAA dispersion exhibited significantly greater cytotoxicity than nTiO 2 without PAA. In addition, the stable nTiO 2 /PAA dispersion induced markedly more oxidative stress than nTiO 2 without PAA. Importantly, the stable nTiO 2 /PAA dispersion caused DNA breakage to a greater extent than nTiO 2 without PAA. Conclusion: Our findings indicated that the anti-coagulant PAA is suitable for preparing homologous dispersed nTiO 2 under realistic physiological culture test conditions.

KW - Cytotoxicity

KW - Dispersing stabilizer

KW - Genotoxicity

KW - Human keratinocytes

KW - Titanium dioxide nanoparticles

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Intrinsic toxicity of stable nanosized titanium dioxide using polyacrylate in human keratinocytes

Abstract

Keywords

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