Investigation of pore size effect on chondrogenic differentiation of adipose stem cells using a pore size gradient scaffold

Se Heang Oh, Tae Ho Kim, Gun Il Im, Jin Ho Lee

Research output: Contribution to journalArticlepeer-review

129 Scopus citations

Abstract

In this study, polycaprolactone (PCL) cylindrical scaffolds with gradually increasing pore size along the longitudinal direction were fabricated by the centrifugation and thermal fibril-bonding process. The fabricated PCL scaffold showed a gradual increasing pore size (from ∼90 to ∼400 μm) and porosity (from ∼80 to ∼97%) along the cylindrical axis. The pore size gradient PCL scaffold was used to investigate the effect of pore size on the chondrogenic differentiation of adipose stem cells (ASCs). From the in vitro culture of ASCs, it was observed that the scaffold section having a pore size range of 370-400 μm provided a more favorable environment for chondrogenic differentiation than other pore size groups. The pore size gradient scaffolds can be a good tool for the systematic study of determining optimum pore size ranges for a variety of stem cell differentiation to a specific cell type.

Original languageEnglish
Pages (from-to)1948-1955
Number of pages8
JournalBiomacromolecules
Volume11
Issue number8
DOIs
StatePublished - 9 Aug 2010

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