Investigation of the one-step electrochemical deposition of graphene oxide-doped poly(3,4-ethylenedioxythiophene)-polyphenol oxidase as a dopamine sensor

P. Ramu, S. P. Vimal, P. Suresh, Anandhavelu Sanmugam, U. Saravanakumar, Raju Suresh Kumar, Abdulrahman I. Almansour, Natarajan Arumugam, Dhanasekaran Vikraman

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

In this paper, we fabricated poly(3,4-ethylenedioxythiophene) (PEDOT)-graphene oxide-polyphenol oxidase (PEDOT-GO-PPO) as a dopamine sensor. The morphology of PEDOT-GO-PPO was observed using scanning electron microscopy. Cyclic voltammetry was conducted to study the oxidation-reduction characteristics of dopamine. To optimize the pH, potential and limit of detection of dopamine, the amperometric technique was employed. The found limit of detection was 8 × 10−9 M, and the linear range was from 5 × 10−8 to 8.5 × 10−5 M. The Michaelis-Menten constant (Km) was calculated to be 70.34 μM, and the activation energy of the prepared electrode was 32.75 kJ mol−1. The electrode shows no significant change in the interference study. The modified electrode retains up to 80% of its original activity after 2 months. In the future, the biosensor can be used for the quantification of dopamine in human urine samples. The present modified electrode constitutes a tool for the electrochemical analysis of dopamine.

Original languageEnglish
Pages (from-to)15575-15583
Number of pages9
JournalRSC Advances
Volume12
Issue number24
DOIs
StatePublished - 23 May 2022

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