Abstract
In this paper, we fabricated poly(3,4-ethylenedioxythiophene) (PEDOT)-graphene oxide-polyphenol oxidase (PEDOT-GO-PPO) as a dopamine sensor. The morphology of PEDOT-GO-PPO was observed using scanning electron microscopy. Cyclic voltammetry was conducted to study the oxidation-reduction characteristics of dopamine. To optimize the pH, potential and limit of detection of dopamine, the amperometric technique was employed. The found limit of detection was 8 × 10−9 M, and the linear range was from 5 × 10−8 to 8.5 × 10−5 M. The Michaelis-Menten constant (Km) was calculated to be 70.34 μM, and the activation energy of the prepared electrode was 32.75 kJ mol−1. The electrode shows no significant change in the interference study. The modified electrode retains up to 80% of its original activity after 2 months. In the future, the biosensor can be used for the quantification of dopamine in human urine samples. The present modified electrode constitutes a tool for the electrochemical analysis of dopamine.
Original language | English |
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Pages (from-to) | 15575-15583 |
Number of pages | 9 |
Journal | RSC Advances |
Volume | 12 |
Issue number | 24 |
DOIs | |
State | Published - 23 May 2022 |