TY - JOUR
T1 - Kinetics of serological response in patients with severe fever with thrombocytopenia syndrome
AU - Ra, Sang Hyun
AU - Kim, Min Jae
AU - Kim, Min Chul
AU - Park, Se Yoon
AU - Park, Seong Yeon
AU - Chong, Yong Pil
AU - Lee, Sang Oh
AU - Choi, Sang Ho
AU - Kim, Yang Soo
AU - Lee, Keun Hwa
AU - Kim, Sung Han
AU - Kee, Sun Ho
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/ licenses/by/4.0/).
PY - 2021/1
Y1 - 2021/1
N2 - Severe fever with thrombocytopenia syndrome (SFTS) is caused by SFTS virus (SFTSV). We investigated the detailed kinetics of serologic response in patients with SFTS. Twenty-eight patients aged ≥18 years were enrolled between July 2015 and October 2018. SFTS was confirmed by detecting SFTSV RNA in their plasma using reverse transcription polymerase chain reaction. SFTSV-specific IgG and IgM were measured using immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). We found that SFTSV-specific IgG was detected at days 5–9 after symptom onset, and its titer was rising during the course of disease. SFTSV-specific IgM titer peaked at around week 2–3 from symptom onset. The SFTSV-specific seropositive rates for days 5–9, 10–14, 15–19, and 20–24 from symptom onset using IFA and ELISA were 63%, 76%, 90%, and 100%, and 58%, 86%, 100%, and 100%, respectively, for IgG, whereas they were 32%, 62%, 80%, and 100%, and 53%, 62%, 70%, and 100%, respectively, for IgM. The delayed IgM response could be attributed to the low sensitivity of SFTSV-specific IgM IFA or ELISA and/or impaired immune responses. The IgM test using IFA or ELISA that we used in this study is, therefore, insufficient for the early diagnosis of SFTS.
AB - Severe fever with thrombocytopenia syndrome (SFTS) is caused by SFTS virus (SFTSV). We investigated the detailed kinetics of serologic response in patients with SFTS. Twenty-eight patients aged ≥18 years were enrolled between July 2015 and October 2018. SFTS was confirmed by detecting SFTSV RNA in their plasma using reverse transcription polymerase chain reaction. SFTSV-specific IgG and IgM were measured using immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). We found that SFTSV-specific IgG was detected at days 5–9 after symptom onset, and its titer was rising during the course of disease. SFTSV-specific IgM titer peaked at around week 2–3 from symptom onset. The SFTSV-specific seropositive rates for days 5–9, 10–14, 15–19, and 20–24 from symptom onset using IFA and ELISA were 63%, 76%, 90%, and 100%, and 58%, 86%, 100%, and 100%, respectively, for IgG, whereas they were 32%, 62%, 80%, and 100%, and 53%, 62%, 70%, and 100%, respectively, for IgM. The delayed IgM response could be attributed to the low sensitivity of SFTSV-specific IgM IFA or ELISA and/or impaired immune responses. The IgM test using IFA or ELISA that we used in this study is, therefore, insufficient for the early diagnosis of SFTS.
KW - Enzyme-linked immunosorbent assay (ELISA)
KW - Immunofluorescence assay (IFA)
KW - Immunoglobulin G (IgG)
KW - Immunoglobulin M (IgM)
KW - Severe fever with thrombocytopenia syndrome (SFTS)
KW - Viral load
UR - http://www.scopus.com/inward/record.url?scp=85099115884&partnerID=8YFLogxK
U2 - 10.3390/v13010006
DO - 10.3390/v13010006
M3 - Article
C2 - 33375753
AN - SCOPUS:85099115884
SN - 1999-4915
VL - 13
JO - Viruses
JF - Viruses
IS - 1
M1 - 6
ER -