Abstract
A new method for quantitative phopholipase activity assays using mass spectrometry (MS) and a supported thin film consisting of a graphene oxide (GO)/carbon nanotube (CNT) double layer as a substrate for laser desorption ionization (LDI) has been developed. Phospholipids were very efficiently analyzed by LDI-time-of-flight (TOF) MS on the GO/CNT films, presumably because of the affinity of phospholipids for the GO/CNT surface. Therefore, the rate of lipid hydrolysis was conveniently measured using LDI-TOF mass spectra obtained from a GO/CNT surface on which the phospholipid hydrolysis reaction mixtures had been spotted by comparing the mass-peak intensities of reactants and products. The present platform for phospholipase assays based on MS and GO/CNT double-layer films enables quantitative measurements at low cost, allows assays to be performed in a short time, and is compatible with an array format, unlike conventional assay methods.
Original language | English |
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Pages (from-to) | 14714-14717 |
Number of pages | 4 |
Journal | Journal of the American Chemical Society |
Volume | 132 |
Issue number | 42 |
DOIs | |
State | Published - 27 Oct 2010 |