LC-UV Analysis of N-{3-(2,4-Dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl}-N- [4-{3-(2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl-amino}butyl]acetamide (SP-8203) in Rat Plasma, Urine, and Gastrointestinal Tract Samples

Young Hee Choi; Jung Hwa Suh; Joo Hyun Lee; Il Hwan Cho; Chul Kyu Lee; Myung Gull Lee

doi:10.1365/s10337-009-1327-1

LC-UV Analysis of N-{3-(2,4-Dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl}-N- [4-{3-(2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl-amino}butyl]acetamide (SP-8203) in Rat Plasma, Urine, and Gastrointestinal Tract Samples

Young Hee Choi, Jung Hwa Suh, Joo Hyun Lee, Il Hwan Cho, Chul Kyu Lee, Myung Gull Lee

Department of Pharmacy

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4 Scopus citations

Abstract

A simple, rapid, and reproducible reversed-phase LC method with UV detection at 215 nm has been developed for analysis of SP-8203 in rat samples. A C₁₈ column was used with 3,000:1,050 (v/v) 0.01 m K ₂HPO₄ buffer (pH 3)-acetonitrile as mobile phase at a flow rate of 1.7 mL min^-1 at 50 °C. Samples were extracted with dichloromethane containing ondansetron (internal standard). Detection limits for SP-8203 in plasma, urine, and gastrointestinal tract samples were 0.05, 0.5, and 10 μg mL^-1, respectively. The method was suitable for pharmacokinetic study of SP-8203 in rats after intravenous administration.

Original language	English
Pages (from-to)	1435-1439
Number of pages	5
Journal	Chromatographia
Volume	70
Issue number	9-10
DOIs	https://doi.org/10.1365/s10337-009-1327-1
State	Published - Nov 2009

Keywords

Column liquid chromatography
Rat plasma, urine, and gastrointestinal tract
SP-8203

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Choi, Y. H., Suh, J. H., Lee, J. H., Cho, I. H., Lee, C. K., & Lee, M. G. (2009). LC-UV Analysis of N-{3-(2,4-Dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl}-N- [4-{3-(2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl-amino}butyl]acetamide (SP-8203) in Rat Plasma, Urine, and Gastrointestinal Tract Samples. Chromatographia, 70(9-10), 1435-1439. https://doi.org/10.1365/s10337-009-1327-1

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title = "LC-UV Analysis of N-{3-(2,4-Dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl}-N- [4-{3-(2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl-amino}butyl]acetamide (SP-8203) in Rat Plasma, Urine, and Gastrointestinal Tract Samples",

abstract = "A simple, rapid, and reproducible reversed-phase LC method with UV detection at 215 nm has been developed for analysis of SP-8203 in rat samples. A C18 column was used with 3,000:1,050 (v/v) 0.01 m K 2HPO4 buffer (pH 3)-acetonitrile as mobile phase at a flow rate of 1.7 mL min-1 at 50 °C. Samples were extracted with dichloromethane containing ondansetron (internal standard). Detection limits for SP-8203 in plasma, urine, and gastrointestinal tract samples were 0.05, 0.5, and 10 μg mL-1, respectively. The method was suitable for pharmacokinetic study of SP-8203 in rats after intravenous administration.",

keywords = "Column liquid chromatography, Rat plasma, urine, and gastrointestinal tract, SP-8203",

author = "Choi, {Young Hee} and Suh, {Jung Hwa} and Lee, {Joo Hyun} and Cho, {Il Hwan} and Lee, {Chul Kyu} and Lee, {Myung Gull}",

year = "2009",

month = nov,

doi = "10.1365/s10337-009-1327-1",

language = "English",

volume = "70",

pages = "1435--1439",

journal = "Chromatographia",

issn = "0009-5893",

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number = "9-10",

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Choi, YH, Suh, JH, Lee, JH, Cho, IH, Lee, CK & Lee, MG 2009, 'LC-UV Analysis of N-{3-(2,4-Dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl}-N- [4-{3-(2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl-amino}butyl]acetamide (SP-8203) in Rat Plasma, Urine, and Gastrointestinal Tract Samples', Chromatographia, vol. 70, no. 9-10, pp. 1435-1439. https://doi.org/10.1365/s10337-009-1327-1

LC-UV Analysis of N-{3-(2,4-Dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl}-N- [4-{3-(2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl-amino}butyl]acetamide (SP-8203) in Rat Plasma, Urine, and Gastrointestinal Tract Samples. / Choi, Young Hee; Suh, Jung Hwa; Lee, Joo Hyun et al.
In: Chromatographia, Vol. 70, No. 9-10, 11.2009, p. 1435-1439.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - LC-UV Analysis of N-{3-(2,4-Dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl}-N- [4-{3-(2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl-amino}butyl]acetamide (SP-8203) in Rat Plasma, Urine, and Gastrointestinal Tract Samples

AU - Choi, Young Hee

AU - Suh, Jung Hwa

AU - Lee, Joo Hyun

AU - Cho, Il Hwan

AU - Lee, Chul Kyu

AU - Lee, Myung Gull

PY - 2009/11

Y1 - 2009/11

N2 - A simple, rapid, and reproducible reversed-phase LC method with UV detection at 215 nm has been developed for analysis of SP-8203 in rat samples. A C18 column was used with 3,000:1,050 (v/v) 0.01 m K 2HPO4 buffer (pH 3)-acetonitrile as mobile phase at a flow rate of 1.7 mL min-1 at 50 °C. Samples were extracted with dichloromethane containing ondansetron (internal standard). Detection limits for SP-8203 in plasma, urine, and gastrointestinal tract samples were 0.05, 0.5, and 10 μg mL-1, respectively. The method was suitable for pharmacokinetic study of SP-8203 in rats after intravenous administration.

AB - A simple, rapid, and reproducible reversed-phase LC method with UV detection at 215 nm has been developed for analysis of SP-8203 in rat samples. A C18 column was used with 3,000:1,050 (v/v) 0.01 m K 2HPO4 buffer (pH 3)-acetonitrile as mobile phase at a flow rate of 1.7 mL min-1 at 50 °C. Samples were extracted with dichloromethane containing ondansetron (internal standard). Detection limits for SP-8203 in plasma, urine, and gastrointestinal tract samples were 0.05, 0.5, and 10 μg mL-1, respectively. The method was suitable for pharmacokinetic study of SP-8203 in rats after intravenous administration.

KW - Column liquid chromatography

KW - Rat plasma, urine, and gastrointestinal tract

KW - SP-8203

UR - http://www.scopus.com/inward/record.url?scp=72949101435&partnerID=8YFLogxK

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DO - 10.1365/s10337-009-1327-1

M3 - Article

AN - SCOPUS:72949101435

SN - 0009-5893

VL - 70

SP - 1435

EP - 1439

JO - Chromatographia

JF - Chromatographia

IS - 9-10

ER -

LC-UV Analysis of N-{3-(2,4-Dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl}-N- [4-{3-(2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl-amino}butyl]acetamide (SP-8203) in Rat Plasma, Urine, and Gastrointestinal Tract Samples

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