TY - JOUR
T1 - Lignans with NADPH oxidase 2 (NOX2)-inhibitory activity from the fruits of Schisandra chinensis
AU - Park, Jung Min
AU - Pel, Pisey
AU - Chin, Young Won
AU - Lee, Moo Yeol
N1 - Publisher Copyright:
© 2018, Korean Society of Pharmacognosy. All rights reserved.
PY - 2018
Y1 - 2018
N2 - An isoform of NADPH oxidase (NOX), NOX2 is a superoxide-generating enzyme involved in diverse pathophysiological events. Although its potential as a therapeutic target has been validated, there is no clinically available inhibitor. Herein, NOX2-inhibitory activity was screened with the constituents isolated from Schisandra chinensis, which has been reported to have antioxidant and reactive oxygen species (ROS)-scavenging effects. Among the partitions prepared from crude methanolic extract, a chloroform-soluble partition showed the highest NOX2-inhibitory activity in PLB-985 cell-based NOX2 assay. A total of twenty nine compounds (1 - 29) were identified from the chloroform fraction, including two first isolated compounds; dimethyl-malate (25) and 2-(2-hydroxyacetyl) furan (27) from this plants. Of these constituents, two compounds (gomisin T, and pregomisin) exhibited an NOX2-inhibitory effect with the IC50 of 9.4 ± 3.6, and 62.9 ± 11.3 µM, respectively. They are confirmed not to be nonspecific superoxide scavengers in a counter assay using a xanthine–xanthine oxidase system. These findings suggest the potential application of gomisin T (6) and other constituents of S. chinensis to inhibit NOX2.
AB - An isoform of NADPH oxidase (NOX), NOX2 is a superoxide-generating enzyme involved in diverse pathophysiological events. Although its potential as a therapeutic target has been validated, there is no clinically available inhibitor. Herein, NOX2-inhibitory activity was screened with the constituents isolated from Schisandra chinensis, which has been reported to have antioxidant and reactive oxygen species (ROS)-scavenging effects. Among the partitions prepared from crude methanolic extract, a chloroform-soluble partition showed the highest NOX2-inhibitory activity in PLB-985 cell-based NOX2 assay. A total of twenty nine compounds (1 - 29) were identified from the chloroform fraction, including two first isolated compounds; dimethyl-malate (25) and 2-(2-hydroxyacetyl) furan (27) from this plants. Of these constituents, two compounds (gomisin T, and pregomisin) exhibited an NOX2-inhibitory effect with the IC50 of 9.4 ± 3.6, and 62.9 ± 11.3 µM, respectively. They are confirmed not to be nonspecific superoxide scavengers in a counter assay using a xanthine–xanthine oxidase system. These findings suggest the potential application of gomisin T (6) and other constituents of S. chinensis to inhibit NOX2.
KW - Gomisin T
KW - NADPH oxidase 2 (NOX2)
KW - NOX2 inhibitor
KW - Schisandra chinensis
UR - http://www.scopus.com/inward/record.url?scp=85045692393&partnerID=8YFLogxK
U2 - 10.20307/nps.2018.24.1.59
DO - 10.20307/nps.2018.24.1.59
M3 - Article
AN - SCOPUS:85045692393
SN - 1226-3907
VL - 24
SP - 59
EP - 65
JO - Natural Product Sciences
JF - Natural Product Sciences
IS - 1
ER -