Lignans with NADPH oxidase 2 (NOX2)-inhibitory activity from the fruits of Schisandra chinensis

Jung Min Park, Pisey Pel, Young Won Chin, Moo Yeol Lee

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

An isoform of NADPH oxidase (NOX), NOX2 is a superoxide-generating enzyme involved in diverse pathophysiological events. Although its potential as a therapeutic target has been validated, there is no clinically available inhibitor. Herein, NOX2-inhibitory activity was screened with the constituents isolated from Schisandra chinensis, which has been reported to have antioxidant and reactive oxygen species (ROS)-scavenging effects. Among the partitions prepared from crude methanolic extract, a chloroform-soluble partition showed the highest NOX2-inhibitory activity in PLB-985 cell-based NOX2 assay. A total of twenty nine compounds (1 - 29) were identified from the chloroform fraction, including two first isolated compounds; dimethyl-malate (25) and 2-(2-hydroxyacetyl) furan (27) from this plants. Of these constituents, two compounds (gomisin T, and pregomisin) exhibited an NOX2-inhibitory effect with the IC50 of 9.4 ± 3.6, and 62.9 ± 11.3 µM, respectively. They are confirmed not to be nonspecific superoxide scavengers in a counter assay using a xanthine–xanthine oxidase system. These findings suggest the potential application of gomisin T (6) and other constituents of S. chinensis to inhibit NOX2.

Original languageEnglish
Pages (from-to)59-65
Number of pages7
JournalNatural Product Sciences
Volume24
Issue number1
DOIs
StatePublished - 2018

Keywords

  • Gomisin T
  • NADPH oxidase 2 (NOX2)
  • NOX2 inhibitor
  • Schisandra chinensis

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