Abstract
Abnormal degradation of matrix components due to dysregulated expression of matrix metalloproteinase (MMP)-9 in macrophages has been linked to progression of acute cerebral ischemia and atherosclerosis. We report that lithium chloride (LiCl) or CHIR99021, inhibitors of Wnt signaling pathway, enhance phosphorylation of glycogen synthase kinase-3beta and suppress lipopolysaccharide-mediated upregulation of MMP-9 expression in murine macrophage RAW264.7 cells in a dosedependent manner. Suppression of MMP-9 expression by LiCl or CHIR99021 did not result after inhibition of kinases involved in NFκB or AP-1 family proteins, but from changes in the activity of histone deacetylases. Beneficial effects on atherosclerosis or cerebral ischemia in animal studies caused by LiCl may be in part explained by the suppression of MMP-9 gene expression.
Original language | English |
---|---|
Pages (from-to) | 177-184 |
Number of pages | 8 |
Journal | Cell Biology International |
Volume | 39 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2015 |
Keywords
- GSK-3b
- HDAC
- Lipopolysaccharide
- Macrophage
- MMP-9
- MMP-9