Lithium chloride suppresses LPS-mediated matrix metalloproteinase-9 expression in macrophages through phosphorylation of GSK-3b

Sooho Kim, Naeun Bong, Ok Soo Kim, Jun Yup Jin, Dong Eog Kim, Dong Kun Lee

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Abnormal degradation of matrix components due to dysregulated expression of matrix metalloproteinase (MMP)-9 in macrophages has been linked to progression of acute cerebral ischemia and atherosclerosis. We report that lithium chloride (LiCl) or CHIR99021, inhibitors of Wnt signaling pathway, enhance phosphorylation of glycogen synthase kinase-3beta and suppress lipopolysaccharide-mediated upregulation of MMP-9 expression in murine macrophage RAW264.7 cells in a dosedependent manner. Suppression of MMP-9 expression by LiCl or CHIR99021 did not result after inhibition of kinases involved in NFκB or AP-1 family proteins, but from changes in the activity of histone deacetylases. Beneficial effects on atherosclerosis or cerebral ischemia in animal studies caused by LiCl may be in part explained by the suppression of MMP-9 gene expression.

Original languageEnglish
Pages (from-to)177-184
Number of pages8
JournalCell Biology International
Volume39
Issue number2
DOIs
StatePublished - Feb 2015

Keywords

  • GSK-3b
  • HDAC
  • Lipopolysaccharide
  • Macrophage
  • MMP-9
  • MMP-9

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