Liver carcinogenesis and formation of 8-hydroxy-deoxyguanosine in C3H/HeN mice by oxidized dietary oils containing carcinogenic dicarbonyl compounds

T. Ichinose, S. Nobuyuki, H. Takano, M. Abe, K. Sadakane, R. Yanagisawa, H. Ochi, K. Fujioka, K. G. Lee, T. Shibamoto

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25 Scopus citations

Abstract

Oxidized dietary oils (lard, soybean oil, and sardine oil) were orally administered to C3H/HeN male mice. After 6 months, benign hepatocellular adenoma was observed in the mice treated with all three oxidized dietary oils. After 12 months, malignant hepatocellular carcinoma and hepatoblastoma were observed in addition to the benign tumor. Oxidized sardine oil caused the highest tumor incidence (35%) and malignant tumors (27.5%) among the oxidized dietary oils tested. Mice treated with oxidized lard and sardine oil exhibited a significant increase of 8-OH-dG in the livers. The amounts of 8-OH-dG found in the mice treated with oxidized sardine oil correlated with the rates of tumor incidence. After 6 months, mRNA decreased in the case of oxidized lard and sardine oil, whereas it increased in the case of oxidized soybean oil, either in 8-oxoguanine-DNA glycosylase (OGG1) or in 8-oxo-dGTPase. On the other hand, there was no appreciable change in mRNA, in either OGG1 or 8-oxo-dGTPase, after 12 months. Oxidized sardine oil contained the highest level of malonaldehyde (MA) (713 ± 91.1 nmol/g) and glyoxal (33.3 ± 5.2 nmol/g) among three oxidized oils. The malignant tumor incidence correlated with the high level of MA and glyoxal found in the dietary oils tested.

Original languageEnglish
Pages (from-to)1795-1803
Number of pages9
JournalFood and Chemical Toxicology
Volume42
Issue number11
DOIs
StatePublished - Nov 2004

Keywords

  • 8-Hydroxy-deoxyguanosine
  • C3H/MeN mice
  • Glyoxal
  • Liver cancer
  • Malonaldehyde
  • Oxidized dietary oils

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