TY - JOUR
T1 - Low-strength ultrasonication positively affects methanogenic granules toward higher AD performance
T2 - Hydrolytic enzyme excretions
AU - Cho, Si Kyung
AU - Yun, Yeo Myeong
AU - Shin, Seung Gu
N1 - Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2017/5/1
Y1 - 2017/5/1
N2 - In our previous studies, enhanced methane (CH4) production using low-strength ultrasonication was achieved and the results were evidenced by physico-chemical and molecular biological approaches. As a final continuation study, the effects of low-strength ultrasonication on the activities of hydrolytic enzymes (amylase, cellulase, and protease) were investigated on methanogenic granules given that hydrolysis regulates the whole anaerobic digestion (AD) process. Up to 213% enhanced hydrolytic enzyme activities were observed, and they seem to be highly related to the enhanced CH4 production. However, the effects of ultrasonication on the distribution (liquid- and solid-phases) of hydrolytic enzymes were negligible. Enzymatic activation by low-strength ultrasonication was hypothetically caused by acoustic streaming, presumably enabling to overcome the masking effect, substrate inhibition and spatial constraint.
AB - In our previous studies, enhanced methane (CH4) production using low-strength ultrasonication was achieved and the results were evidenced by physico-chemical and molecular biological approaches. As a final continuation study, the effects of low-strength ultrasonication on the activities of hydrolytic enzymes (amylase, cellulase, and protease) were investigated on methanogenic granules given that hydrolysis regulates the whole anaerobic digestion (AD) process. Up to 213% enhanced hydrolytic enzyme activities were observed, and they seem to be highly related to the enhanced CH4 production. However, the effects of ultrasonication on the distribution (liquid- and solid-phases) of hydrolytic enzymes were negligible. Enzymatic activation by low-strength ultrasonication was hypothetically caused by acoustic streaming, presumably enabling to overcome the masking effect, substrate inhibition and spatial constraint.
KW - Hydrolytic enzyme
KW - Low-strength ultrasonication
KW - Methanogenic granule
KW - Upflow anaerobic sludge blanket
UR - http://www.scopus.com/inward/record.url?scp=85000819067&partnerID=8YFLogxK
U2 - 10.1016/j.ultsonch.2016.11.023
DO - 10.1016/j.ultsonch.2016.11.023
M3 - Article
C2 - 28069197
AN - SCOPUS:85000819067
SN - 1350-4177
VL - 36
SP - 168
EP - 172
JO - Ultrasonics Sonochemistry
JF - Ultrasonics Sonochemistry
ER -