TY - JOUR
T1 - Lysobacter humi sp. Nov., isolated from soil
AU - Lee, Dongwook
AU - Jang, Jun Hyeong
AU - Cha, Seho
AU - Seo, Taegun
N1 - Publisher Copyright:
© 2017 IUMS.
PY - 2017/4
Y1 - 2017/4
N2 - A yellow-pigmented and strictly aerobic bacterial strain, designated FJY8T, was isolated from the soil of Goyang, South Korea. The cells of FJY8Twere Gram-reaction-negative, non-motile rods. Colonies were circular, convex and transparent. Strain FJY8Tgrew optimally at 30 °C, with 0 % (w/v) NaCl and at pH 8. Phylogenetic analysis of the 16S rRNA gene sequence of FJY8Trevealed a clear affiliation of this bacterium to the family Lysobacteraceae, and it was related to members of the genus Lysobacter, with Lysobacter xinjiangensis KCTC 22558Tbeing its closest relative (98.7 % sequence similarity). The DNA G+C content was 68.0±0.4 mol%. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were identified as the major polar lipids, and an unidentified phospholipid and two unidentified aminophospholipids were also detected as the minor polar lipids. The major fatty acids were iso-C16 : 0, summed feature 9 (iso-C17 : 1ω9c and/or C16 : 010-methyl) and iso-C15 : 0. Only ubiquinone-8 (Q-8) was detected as the isoprenoid quinone. DNA–DNA hybridization values of strain FJY8Twith Lysobacter xinjiangensis RCML-52Tand Lysobacter mobilis 9NM-14Twere 55.8±2.0 and 45.2±4.8 %, respectively. On the basis of DNA–DNA hybridization, phylogenetic distinctiveness, and some physiological and biochemical tests, strain FJY8T(=KCTC 42810T=JCM 31019T) represents a novel species of the genus Lysobacter, for which the name Lysobacter humi sp. nov. is proposed.
AB - A yellow-pigmented and strictly aerobic bacterial strain, designated FJY8T, was isolated from the soil of Goyang, South Korea. The cells of FJY8Twere Gram-reaction-negative, non-motile rods. Colonies were circular, convex and transparent. Strain FJY8Tgrew optimally at 30 °C, with 0 % (w/v) NaCl and at pH 8. Phylogenetic analysis of the 16S rRNA gene sequence of FJY8Trevealed a clear affiliation of this bacterium to the family Lysobacteraceae, and it was related to members of the genus Lysobacter, with Lysobacter xinjiangensis KCTC 22558Tbeing its closest relative (98.7 % sequence similarity). The DNA G+C content was 68.0±0.4 mol%. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were identified as the major polar lipids, and an unidentified phospholipid and two unidentified aminophospholipids were also detected as the minor polar lipids. The major fatty acids were iso-C16 : 0, summed feature 9 (iso-C17 : 1ω9c and/or C16 : 010-methyl) and iso-C15 : 0. Only ubiquinone-8 (Q-8) was detected as the isoprenoid quinone. DNA–DNA hybridization values of strain FJY8Twith Lysobacter xinjiangensis RCML-52Tand Lysobacter mobilis 9NM-14Twere 55.8±2.0 and 45.2±4.8 %, respectively. On the basis of DNA–DNA hybridization, phylogenetic distinctiveness, and some physiological and biochemical tests, strain FJY8T(=KCTC 42810T=JCM 31019T) represents a novel species of the genus Lysobacter, for which the name Lysobacter humi sp. nov. is proposed.
KW - DNA-DNA relatedness
KW - Lysobacter
KW - Taxonomy
UR - http://www.scopus.com/inward/record.url?scp=85019016377&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.001722
DO - 10.1099/ijsem.0.001722
M3 - Article
C2 - 27930269
AN - SCOPUS:85019016377
SN - 1466-5026
VL - 67
SP - 951
EP - 955
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 4
M1 - 001722
ER -