Mass spectrometric determination of Zn2+ binding/dissociation constant for zinc finger peptides

Choong Sik Lee; Soo Jin Park; Jae Young Lee; Sungsu Park; Kyubong Jo; Han Bin Oh

doi:10.5478/MSL.2015.6.1.7

Mass spectrometric determination of Zn²⁺ binding/dissociation constant for zinc finger peptides

Choong Sik Lee, Soo Jin Park, Jae Young Lee, Sungsu Park, Kyubong Jo, Han Bin Oh

Department of Life Science

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

In the present study, we proposed a simple ESI-MS model for determining Zn²⁺binding (or dissociation) constants for zinc finger peptides (ZFPs) with a unique ββα fold consensus. The ionization efficiency (response) factors for this model, i.e., α and β, could be determined for ZiCo ZFP with a known Zn²⁺binding constant. We could determine the binding constants for other ZFPs assuming those with a ββα consensus conformation have the same α/β response ratio. In general, the ZPF dissociation constants exhibited K_d values of 10^-7~10^-9M, while K_d values for a negative control non-specific Zn²⁺peptides were high, e.g., 5.5×10^-6M and 4.3×10^-4M for BBA1 and melittin, respectively.

Original language	English
Pages (from-to)	7-12
Number of pages	6
Journal	Mass Spectrometry Letters
Volume	6
Issue number	1
DOIs	https://doi.org/10.5478/MSL.2015.6.1.7
State	Published - 1 Mar 2015

Keywords

Binding constant
Electrospray-mass spectrometry
Zinc finger
Zinc ion

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abstract = "In the present study, we proposed a simple ESI-MS model for determining Zn2+binding (or dissociation) constants for zinc finger peptides (ZFPs) with a unique ββα fold consensus. The ionization efficiency (response) factors for this model, i.e., α and β, could be determined for ZiCo ZFP with a known Zn2+binding constant. We could determine the binding constants for other ZFPs assuming those with a ββα consensus conformation have the same α/β response ratio. In general, the ZPF dissociation constants exhibited Kd values of 10-7~10-9M, while Kd values for a negative control non-specific Zn2+peptides were high, e.g., 5.5×10-6M and 4.3×10-4M for BBA1 and melittin, respectively.",

keywords = "Binding constant, Electrospray-mass spectrometry, Zinc finger, Zinc ion",

author = "Lee, {Choong Sik} and Park, {Soo Jin} and Lee, {Jae Young} and Sungsu Park and Kyubong Jo and Oh, {Han Bin}",

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T1 - Mass spectrometric determination of Zn2+ binding/dissociation constant for zinc finger peptides

AU - Lee, Choong Sik

AU - Park, Soo Jin

AU - Lee, Jae Young

AU - Park, Sungsu

AU - Jo, Kyubong

AU - Oh, Han Bin

PY - 2015/3/1

Y1 - 2015/3/1

N2 - In the present study, we proposed a simple ESI-MS model for determining Zn2+binding (or dissociation) constants for zinc finger peptides (ZFPs) with a unique ββα fold consensus. The ionization efficiency (response) factors for this model, i.e., α and β, could be determined for ZiCo ZFP with a known Zn2+binding constant. We could determine the binding constants for other ZFPs assuming those with a ββα consensus conformation have the same α/β response ratio. In general, the ZPF dissociation constants exhibited Kd values of 10-7~10-9M, while Kd values for a negative control non-specific Zn2+peptides were high, e.g., 5.5×10-6M and 4.3×10-4M for BBA1 and melittin, respectively.

AB - In the present study, we proposed a simple ESI-MS model for determining Zn2+binding (or dissociation) constants for zinc finger peptides (ZFPs) with a unique ββα fold consensus. The ionization efficiency (response) factors for this model, i.e., α and β, could be determined for ZiCo ZFP with a known Zn2+binding constant. We could determine the binding constants for other ZFPs assuming those with a ββα consensus conformation have the same α/β response ratio. In general, the ZPF dissociation constants exhibited Kd values of 10-7~10-9M, while Kd values for a negative control non-specific Zn2+peptides were high, e.g., 5.5×10-6M and 4.3×10-4M for BBA1 and melittin, respectively.

KW - Binding constant

KW - Electrospray-mass spectrometry

KW - Zinc finger

KW - Zinc ion

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Mass spectrometric determination of Zn²⁺ binding/dissociation constant for zinc finger peptides

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Keywords

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