Membrane depolarization stimulates the proliferation of SH-SY5Y human neuroblastoma cells by increasing retinoblastoma protein (RB) phosphorylation through the activation of cyclin-dependent kinase 2 (Cdk2)

Miran Seo, Yeni Kim, Yun Il Lee, So Young Kim, Yong Min Ahn, Ung Gu Kang, Myoung Sun Roh, Yong Sik Kim, Yong Sung Juhnn

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Membrane depolarization causes transmembrane ionic influxes that induce various gene expressions, and is involved in the processes of neuronal differentiation and apoptosis. However, the effect of membrane depolarization on neuronal proliferation has not been established. In this study, we aimed to investigate the effect of membrane depolarization on the proliferation of SH-SY5Y human neuroblastoma cells. Membrane depolarization induced by 50 mM KCl for 5 min significantly increased SH-SY5Y cell numbers and thymidine incorporation at 24 h after depolarization, and increased the phosphorylation and expression of retinoblastoma protein (RB), the activity of Cdk2 (without changing the activities of Cdk4 and Cdk6), and the expressions of cyclin A and cyclin E. Single and repeated depolarization (once a day for 6 days) had similar effects on RB, Cdks, and cyclins levels and activities. In summary, our results suggest that membrane depolarization may stimulate cellular proliferation by augmenting the expression of cyclin E leading to increases in Cdk2 activity and RB phosphorylation.

Original languageEnglish
Pages (from-to)87-92
Number of pages6
JournalNeuroscience Letters
Volume404
Issue number1-2
DOIs
StatePublished - 14 Aug 2006

Keywords

  • Cyclin-dependent kinases
  • Cyclins
  • Membrane depolarization
  • Neuroblastoma cells
  • Proliferation
  • Retinoblastoma protein

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