TY - JOUR
T1 - Metabolic engineering of Enterobacter aerogenes for 2,3-butanediol production from sugarcane bagasse hydrolysate
AU - Um, Jaeyong
AU - Kim, Duck Gyun
AU - Jung, Moo Young
AU - Saratale, Ganesh D.
AU - Oh, Min Kyu
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/12
Y1 - 2017/12
N2 - The pathway engineering of Enterobacter aerogenes was attempted to improve its production capability of 2,3–butanediol from lignocellulosic biomass. In the medium containing glucose and xylose mixture as carbon sources, the gene deletion of pflB improved 2,3-butanediol carbon yield by 40%, while the deletion of ptsG increased xylose consumption rate significantly, improving the productivity at 12 hr by 70%. The constructed strain, EMY-22-galP, overexpressing glucose transporter (galP) in the triple gene knockout E. aerogenes, ldhA, pflB, and ptsG, provided the highest 2,3-butanediol titer and yield at 12 hr flask cultivation. Sugarcane bagasse was pretreated with green liquor, a solution containing Na2CO3 and Na2SO3 and was hydrolyzed by enzymes. The resulting hydrolysate was used as a carbon source for 2,3-butanediol production. After 72 hr in fermentation, the yield of 0.395 g/g sugar was achieved, suggesting an economic production of 2,3-butanediol was possible from lignocellulosic biomass with the metabolically engineered strain.
AB - The pathway engineering of Enterobacter aerogenes was attempted to improve its production capability of 2,3–butanediol from lignocellulosic biomass. In the medium containing glucose and xylose mixture as carbon sources, the gene deletion of pflB improved 2,3-butanediol carbon yield by 40%, while the deletion of ptsG increased xylose consumption rate significantly, improving the productivity at 12 hr by 70%. The constructed strain, EMY-22-galP, overexpressing glucose transporter (galP) in the triple gene knockout E. aerogenes, ldhA, pflB, and ptsG, provided the highest 2,3-butanediol titer and yield at 12 hr flask cultivation. Sugarcane bagasse was pretreated with green liquor, a solution containing Na2CO3 and Na2SO3 and was hydrolyzed by enzymes. The resulting hydrolysate was used as a carbon source for 2,3-butanediol production. After 72 hr in fermentation, the yield of 0.395 g/g sugar was achieved, suggesting an economic production of 2,3-butanediol was possible from lignocellulosic biomass with the metabolically engineered strain.
KW - 2,3-Butanediol
KW - Enterobacter aerogenes
KW - Metabolic engineering
KW - Sugarcane bagasse
UR - http://www.scopus.com/inward/record.url?scp=85020232848&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2017.05.166
DO - 10.1016/j.biortech.2017.05.166
M3 - Article
C2 - 28596073
AN - SCOPUS:85020232848
SN - 0960-8524
VL - 245
SP - 1567
EP - 1574
JO - Bioresource Technology
JF - Bioresource Technology
ER -