Methylobacterium durans sp. nov., a radiation-resistant bacterium isolated from gamma ray-irradiated soil

Jiyoun Kim; Geeta Chhetri; Inhyup Kim; Myung Kyum Kim; Taegun Seo

doi:10.1007/s10482-019-01331-2

Methylobacterium durans sp. nov., a radiation-resistant bacterium isolated from gamma ray-irradiated soil

Jiyoun Kim
, Geeta Chhetri
, Inhyup Kim
, Myung Kyum Kim
, Taegun Seo

Department of Life Science

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

A gamma radiation-resistant, Gram-stain negative, oxidase and catalase positive, aerobic, flagellated, rod-shaped, methylotrophic and pink-pigmented bacterial strain designated 17SD2-17^T was isolated from gamma-ray-irradiated soil collected in Korea. The 16S rRNA gene sequence analysis showed that strain 17SD2-17^T is phylogenetically related to Methylobacterium organophilum DSM 760^T (97.6%), Methylobacterium oxalidis 35a^T (97.4%) and Methylobacterium soli YIM 48816^T (97.0%). The G+C content calculated based on the draft genome sequence is 68.7 mol%. The DNA–DNA hybridisation between 17SD2-17^T and its close relatives was found to be less than 40%. The predominant fatty acid was identified as summed feature 8 (C_18:1ω7c and/or C_18:1ω6c) and the major respiratory quinone as Q-10. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. On the basis of the data from phenotypic tests and genotypic differences between strain 17SD2-17^T and its close phylogenetic relatives, strain 17SD2-17^T is concluded to represent a new species belonging to the genus Methylobacterium, for which the name Methylobacterium durans sp. nov. (= KCTC 52908^T = NBRC 112876^T) is proposed.

Original language	English
Pages (from-to)	211-220
Number of pages	10
Journal	Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume	113
Issue number	2
DOIs	https://doi.org/10.1007/s10482-019-01331-2
State	Published - 1 Feb 2020

Keywords

Gamma-ray resistance
Methylobacterium durans
Methylotrophic metabolism
Polyphasic taxonomy

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title = "Methylobacterium durans sp. nov., a radiation-resistant bacterium isolated from gamma ray-irradiated soil",

abstract = "A gamma radiation-resistant, Gram-stain negative, oxidase and catalase positive, aerobic, flagellated, rod-shaped, methylotrophic and pink-pigmented bacterial strain designated 17SD2-17 T was isolated from gamma-ray-irradiated soil collected in Korea. The 16S rRNA gene sequence analysis showed that strain 17SD2-17 T is phylogenetically related to Methylobacterium organophilum DSM 760 T (97.6\%), Methylobacterium oxalidis 35aT (97.4\%) and Methylobacterium soli YIM 48816 T (97.0\%). The G+C content calculated based on the draft genome sequence is 68.7 mol\%. The DNA–DNA hybridisation between 17SD2-17 T and its close relatives was found to be less than 40\%. The predominant fatty acid was identified as summed feature 8 (C18:1ω7c and/or C18:1ω6c) and the major respiratory quinone as Q-10. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. On the basis of the data from phenotypic tests and genotypic differences between strain 17SD2-17 T and its close phylogenetic relatives, strain 17SD2-17 T is concluded to represent a new species belonging to the genus Methylobacterium, for which the name Methylobacterium durans sp. nov. (= KCTC 52908 T = NBRC 112876 T) is proposed.",

keywords = "Gamma-ray resistance, Methylobacterium durans, Methylotrophic metabolism, Polyphasic taxonomy",

author = "Jiyoun Kim and Geeta Chhetri and Inhyup Kim and Kim, \{Myung Kyum\} and Taegun Seo",

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T1 - Methylobacterium durans sp. nov., a radiation-resistant bacterium isolated from gamma ray-irradiated soil

AU - Kim, Jiyoun

AU - Chhetri, Geeta

AU - Kim, Inhyup

AU - Kim, Myung Kyum

AU - Seo, Taegun

PY - 2020/2/1

Y1 - 2020/2/1

N2 - A gamma radiation-resistant, Gram-stain negative, oxidase and catalase positive, aerobic, flagellated, rod-shaped, methylotrophic and pink-pigmented bacterial strain designated 17SD2-17 T was isolated from gamma-ray-irradiated soil collected in Korea. The 16S rRNA gene sequence analysis showed that strain 17SD2-17 T is phylogenetically related to Methylobacterium organophilum DSM 760 T (97.6%), Methylobacterium oxalidis 35aT (97.4%) and Methylobacterium soli YIM 48816 T (97.0%). The G+C content calculated based on the draft genome sequence is 68.7 mol%. The DNA–DNA hybridisation between 17SD2-17 T and its close relatives was found to be less than 40%. The predominant fatty acid was identified as summed feature 8 (C18:1ω7c and/or C18:1ω6c) and the major respiratory quinone as Q-10. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. On the basis of the data from phenotypic tests and genotypic differences between strain 17SD2-17 T and its close phylogenetic relatives, strain 17SD2-17 T is concluded to represent a new species belonging to the genus Methylobacterium, for which the name Methylobacterium durans sp. nov. (= KCTC 52908 T = NBRC 112876 T) is proposed.

AB - A gamma radiation-resistant, Gram-stain negative, oxidase and catalase positive, aerobic, flagellated, rod-shaped, methylotrophic and pink-pigmented bacterial strain designated 17SD2-17 T was isolated from gamma-ray-irradiated soil collected in Korea. The 16S rRNA gene sequence analysis showed that strain 17SD2-17 T is phylogenetically related to Methylobacterium organophilum DSM 760 T (97.6%), Methylobacterium oxalidis 35aT (97.4%) and Methylobacterium soli YIM 48816 T (97.0%). The G+C content calculated based on the draft genome sequence is 68.7 mol%. The DNA–DNA hybridisation between 17SD2-17 T and its close relatives was found to be less than 40%. The predominant fatty acid was identified as summed feature 8 (C18:1ω7c and/or C18:1ω6c) and the major respiratory quinone as Q-10. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. On the basis of the data from phenotypic tests and genotypic differences between strain 17SD2-17 T and its close phylogenetic relatives, strain 17SD2-17 T is concluded to represent a new species belonging to the genus Methylobacterium, for which the name Methylobacterium durans sp. nov. (= KCTC 52908 T = NBRC 112876 T) is proposed.

KW - Gamma-ray resistance

KW - Methylobacterium durans

KW - Methylotrophic metabolism

KW - Polyphasic taxonomy

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DO - 10.1007/s10482-019-01331-2

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SN - 0003-6072

VL - 113

SP - 211

EP - 220

JO - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

JF - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

IS - 2

ER -

Methylobacterium durans sp. nov., a radiation-resistant bacterium isolated from gamma ray-irradiated soil

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