Methylobacterium terricola sp. Nov., a gamma radiation-resistant bacterium isolated from gamma ray-irradiated soil

Jiyoun Kim, Geeta Chhetri, Inhyup Kim, Byungjo Lee, Wonhee Jang, Myung Kyum Kim, Taegun Seo

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

A gamma radiation-resistant and pink-pigmented bacterial strain, designated as 17Sr1-39T, was isolated from a gamma ray-irradiated soil sample collected in the Republic of Korea. Cells were Gram-stain-negative, strictly aerobic, flagellated, asporogenous, rod-shaped and methylotrophic. Results of 16S rRNA gene sequence analysis showed that strain 17Sr1-39T was phylogenetically related to Methylobacterium currus PR1016AT (97.3%), Methylobacterium aquaticum DSM 16371T (97.2%), Methylobacterium platani PMB02T (97.0%), Methylobacterium frigidaeris IER25-16T (96.6%), Methylobacterium terrae 17Sr1-28T (96.6%) and Methylobacterium organophilum JCM 2833T (93.4%). The G+C content calculated based on the genome sequence was 70.4 mol%. The average nucleotide identity and in silico DNA–DNA hybridization values between strain 17Sr1-39T and M. currus, M. aquaticum, M. platani, M. frigidaeris, M. terrae and M. organophilum were 77.3–89.9 and 22–38.2 %, respectively. The predominant fatty acids were summed feature 8 (C18: 1ω7c and/or C18: 1ω6c) and summed feature 3 (C16: 1ω7c and/or C16: 1ω6c). The predominant quinone was ubiquinone 10 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Based on the data from phenotypic tests and genotypic differences between strain 17Sr1-39T and its close phylogenetic relatives, strain 17Sr1-39T represented a new species belonging to the genus Methylobacterium, for which the name Methylobacterium terricola sp. nov. (=KACC 52905T=NBRC 112874T) is proposed.

Original languageEnglish
Article number004054
Pages (from-to)2449-2456
Number of pages8
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume70
Issue number4
DOIs
StatePublished - 2020

Keywords

  • Gamma ray resistance
  • Methylobacterium terricola
  • Methylotrophic metabolism
  • Polyphasic taxonomy

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