TY - JOUR
T1 - Methylobacterium terricola sp. Nov., a gamma radiation-resistant bacterium isolated from gamma ray-irradiated soil
AU - Kim, Jiyoun
AU - Chhetri, Geeta
AU - Kim, Inhyup
AU - Lee, Byungjo
AU - Jang, Wonhee
AU - Kim, Myung Kyum
AU - Seo, Taegun
N1 - Publisher Copyright:
© 2020 The Authors.
PY - 2020
Y1 - 2020
N2 - A gamma radiation-resistant and pink-pigmented bacterial strain, designated as 17Sr1-39T, was isolated from a gamma ray-irradiated soil sample collected in the Republic of Korea. Cells were Gram-stain-negative, strictly aerobic, flagellated, asporogenous, rod-shaped and methylotrophic. Results of 16S rRNA gene sequence analysis showed that strain 17Sr1-39T was phylogenetically related to Methylobacterium currus PR1016AT (97.3%), Methylobacterium aquaticum DSM 16371T (97.2%), Methylobacterium platani PMB02T (97.0%), Methylobacterium frigidaeris IER25-16T (96.6%), Methylobacterium terrae 17Sr1-28T (96.6%) and Methylobacterium organophilum JCM 2833T (93.4%). The G+C content calculated based on the genome sequence was 70.4 mol%. The average nucleotide identity and in silico DNA–DNA hybridization values between strain 17Sr1-39T and M. currus, M. aquaticum, M. platani, M. frigidaeris, M. terrae and M. organophilum were 77.3–89.9 and 22–38.2 %, respectively. The predominant fatty acids were summed feature 8 (C18: 1ω7c and/or C18: 1ω6c) and summed feature 3 (C16: 1ω7c and/or C16: 1ω6c). The predominant quinone was ubiquinone 10 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Based on the data from phenotypic tests and genotypic differences between strain 17Sr1-39T and its close phylogenetic relatives, strain 17Sr1-39T represented a new species belonging to the genus Methylobacterium, for which the name Methylobacterium terricola sp. nov. (=KACC 52905T=NBRC 112874T) is proposed.
AB - A gamma radiation-resistant and pink-pigmented bacterial strain, designated as 17Sr1-39T, was isolated from a gamma ray-irradiated soil sample collected in the Republic of Korea. Cells were Gram-stain-negative, strictly aerobic, flagellated, asporogenous, rod-shaped and methylotrophic. Results of 16S rRNA gene sequence analysis showed that strain 17Sr1-39T was phylogenetically related to Methylobacterium currus PR1016AT (97.3%), Methylobacterium aquaticum DSM 16371T (97.2%), Methylobacterium platani PMB02T (97.0%), Methylobacterium frigidaeris IER25-16T (96.6%), Methylobacterium terrae 17Sr1-28T (96.6%) and Methylobacterium organophilum JCM 2833T (93.4%). The G+C content calculated based on the genome sequence was 70.4 mol%. The average nucleotide identity and in silico DNA–DNA hybridization values between strain 17Sr1-39T and M. currus, M. aquaticum, M. platani, M. frigidaeris, M. terrae and M. organophilum were 77.3–89.9 and 22–38.2 %, respectively. The predominant fatty acids were summed feature 8 (C18: 1ω7c and/or C18: 1ω6c) and summed feature 3 (C16: 1ω7c and/or C16: 1ω6c). The predominant quinone was ubiquinone 10 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Based on the data from phenotypic tests and genotypic differences between strain 17Sr1-39T and its close phylogenetic relatives, strain 17Sr1-39T represented a new species belonging to the genus Methylobacterium, for which the name Methylobacterium terricola sp. nov. (=KACC 52905T=NBRC 112874T) is proposed.
KW - Gamma ray resistance
KW - Methylobacterium terricola
KW - Methylotrophic metabolism
KW - Polyphasic taxonomy
UR - http://www.scopus.com/inward/record.url?scp=85084254660&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.004054
DO - 10.1099/ijsem.0.004054
M3 - Article
C2 - 32100693
AN - SCOPUS:85084254660
SN - 1466-5026
VL - 70
SP - 2449
EP - 2456
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 4
M1 - 004054
ER -