Abstract
Mild hyperthermia is known to enhance apoptosis. The p53 tumor- suppressor gene product has been shown to function in apoptosis in response to genotoxic stress. However, there is little information regarding the mechanism of p53-dependent apoptosis induced by heat stress. In present study, a p53 contribution in mild hyperthermia-induced apoptosis was investigated in human lymphoid system. After 30-minute exposure at 44°C, the accumulation of p53 protein was clearly observed in TK6 and ML-1 cells. Using comet assay, the more significant sensitivity to hyperthermic apoptosis was found in TK6 (wild-type p53) than in WI-L2-NS (mutated in p53). Furthermore, the significantly rapid shifting from early apoptotic phase to late apoptotic was observed in heat-induced p53 TK6 cells. These findings suggest that p53- dependent apoptosis is efficaciously induced by mild hyperthermia as non- genotoxic stress in human lymphoid system.
Original language | English |
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Pages (from-to) | 285-292 |
Number of pages | 8 |
Journal | Research Communications in Molecular Pathology and Pharmacology |
Volume | 104 |
Issue number | 3 |
State | Published - 1999 |