Modulatory effects of the fruits of Tribulus terrestris L. on the function of atopic dermatitis-related calcium channels, Orai1 and TRPV3

Joo Hyun Nam, Hyo Won Jung, Young Won Chin, Woo Kyung Kim, Hyo Sang Bae

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Objective To examine the effects of Tribulus terrestris L. (T. terrestris) extract on the modulation of calcium channels to evaluate its use in topical agents for treatment of atopic dermatitis. Methods The 70% methanol extract of T. terrestris was prepared. Human HEK293T cells with over-expressed calcium release-activated calcium channel protein 1 (Orai1), transient receptor potential vanilloid 1, or transient receptor potential vanilloid 3 (TRPV3) were treated with T. terrestris extract. Modulation of ion channels was measured using a conventional whole-cell patch-clamp technique. Results T. terrestris extract (100 μg/mL) significantly inhibited Orai1 activity in Orai1-stromal interaction molecule 1 co-overexpressed HEK293T cells. In addition, T. terrestris extract significantly increased the TRPV3 activity compared with 2-Aminoethyl diphenylborinate (100 μmol/L), which induces the full activation of TRPV3. Conclusions Our results suggest that T. terrestris extract may have a therapeutic potential for recovery of abnormal skin barrier pathologies in atopic dermatitis through modulating the activities of calcium ion channels, Orai1 and TRPV3. This is the first study to report the modulatory effect of a medicinal plant on the function of ion channels in skin barrier.

Original languageEnglish
Pages (from-to)580-585
Number of pages6
JournalAsian Pacific Journal of Tropical Biomedicine
Volume6
Issue number7
DOIs
StatePublished - 1 Jul 2016

Keywords

  • Atopic dermatitis
  • Ion channels
  • ORAI1
  • Tribulus terrestris L
  • TRPV3

Fingerprint

Dive into the research topics of 'Modulatory effects of the fruits of Tribulus terrestris L. on the function of atopic dermatitis-related calcium channels, Orai1 and TRPV3'. Together they form a unique fingerprint.

Cite this