Network-based toxicogenomic approach to explore oral benzo(a)pyrene exposure effect on respiratory system

Su Ji Kim, Dong Yeop Shin, Yujin Jang, Sang Min Lee, Cheol Min Lee, Yeon Soon Ahn, Young Rok Seo

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Background: Benzo(a)pyrene is known as a ubiquitous environmental pollutant. The general population can be exposed to benzo(a)pyrene through ambient air, tobacco smoke, water, and food. Genotoxicity and carcinogenicity are well-known adverse effects of benzo(a)pyrene exposure. However, few studies have focused on the effects of oral benzo(a)pyrene exposure on the respiratory system in terms of gene expression profiling. Objectives: We explored the effect of benzo(a)pyrene on respiratory organs through bioinformatics analysis using public gene expression data. Key genes associated with respiratory toxicity were selected through network analysis and validated by Western blot and qRT-PCR. Results: NRF2-mediated oxidative stress response and CREB signaling in neurons were identified as the main canonical pathways related to benzo(a)pyrene toxicity in the respiratory system. Potential biomarkers were validated in human lung cells. Conclusion: Our study suggested canonical pathways related to benzo(a)pyrene toxicity in the respiratory system and validated potential biomarkers in human lung cells.

Original languageEnglish
Pages (from-to)521-529
Number of pages9
JournalMolecular and Cellular Toxicology
Volume18
Issue number4
DOIs
StatePublished - Oct 2022

Keywords

  • Benzo(a)pyrene
  • DNA repair
  • Network analysis
  • Oxidative stress

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